A Practical Solution for 77 K Fluorescence Measurements Based on LED Excitation and CCD Array Detector.

A Practical Solution for 77 K Fluorescence Measurements Based on LED Excitation and CCD Array Detector.

The fluorescence emission spectrum of photosynthetic microorganisms at liquid nitrogen temperature (77 K) provides important insights into the organization of the photosynthetic machinery of bacteria and eukaryotes, which cannot be observed at room temperature. Conventionally, to obtain such spectra...

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Main Authors: Jacob Lamb, Kristin Forfang, Martin Hohmann-Marriott
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4503352?pdf=render
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spelling doaj-ed80849eb7904d378ccc81f17836d5ec2020-11-25T01:53:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01107e013225810.1371/journal.pone.0132258A Practical Solution for 77 K Fluorescence Measurements Based on LED Excitation and CCD Array Detector.Jacob LambKristin ForfangMartin Hohmann-MarriottThe fluorescence emission spectrum of photosynthetic microorganisms at liquid nitrogen temperature (77 K) provides important insights into the organization of the photosynthetic machinery of bacteria and eukaryotes, which cannot be observed at room temperature. Conventionally, to obtain such spectra, a large and costly table-top fluorometer is required. Recently portable, reliable, and largely maintenance-free instruments have become available that can be utilized to accomplish a wide variety of spectroscopy-based measurements in photosynthesis research. In this report, we show how to build such an instrument in order to record 77K fluorescence spectra. This instrument consists of a low power monochromatic light-emitting diode (LED), and a portable CCD array based spectrometer. The optical components are coupled together using a fiber optic cable, and a custom made housing that also supports a dewar flask. We demonstrate that this instrument facilitates the reliable determination of chlorophyll fluorescence emission spectra for the cyanobacterium Synechocystis sp. PCC 6803, and the green alga Chlamydomonas reinhardtii.http://europepmc.org/articles/PMC4503352?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Jacob Lamb
Kristin Forfang
Martin Hohmann-Marriott
spellingShingle Jacob Lamb
Kristin Forfang
Martin Hohmann-Marriott
A Practical Solution for 77 K Fluorescence Measurements Based on LED Excitation and CCD Array Detector.
PLoS ONE
author_facet Jacob Lamb
Kristin Forfang
Martin Hohmann-Marriott
author_sort Jacob Lamb
title A Practical Solution for 77 K Fluorescence Measurements Based on LED Excitation and CCD Array Detector.
title_short A Practical Solution for 77 K Fluorescence Measurements Based on LED Excitation and CCD Array Detector.
title_full A Practical Solution for 77 K Fluorescence Measurements Based on LED Excitation and CCD Array Detector.
title_fullStr A Practical Solution for 77 K Fluorescence Measurements Based on LED Excitation and CCD Array Detector.
title_full_unstemmed A Practical Solution for 77 K Fluorescence Measurements Based on LED Excitation and CCD Array Detector.
title_sort practical solution for 77 k fluorescence measurements based on led excitation and ccd array detector.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2015-01-01
description The fluorescence emission spectrum of photosynthetic microorganisms at liquid nitrogen temperature (77 K) provides important insights into the organization of the photosynthetic machinery of bacteria and eukaryotes, which cannot be observed at room temperature. Conventionally, to obtain such spectra, a large and costly table-top fluorometer is required. Recently portable, reliable, and largely maintenance-free instruments have become available that can be utilized to accomplish a wide variety of spectroscopy-based measurements in photosynthesis research. In this report, we show how to build such an instrument in order to record 77K fluorescence spectra. This instrument consists of a low power monochromatic light-emitting diode (LED), and a portable CCD array based spectrometer. The optical components are coupled together using a fiber optic cable, and a custom made housing that also supports a dewar flask. We demonstrate that this instrument facilitates the reliable determination of chlorophyll fluorescence emission spectra for the cyanobacterium Synechocystis sp. PCC 6803, and the green alga Chlamydomonas reinhardtii.
url http://europepmc.org/articles/PMC4503352?pdf=render
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