Rhodamine B increases hypothalamic cell apoptosis and disrupts hormonal balance in rats

• Main Authors: Dewi Ratna Sulistina, Retty Ratnawati, I Wayan Arsana Wiyasa
• Format: Article
• Language: English
• Published: Wolters Kluwer Medknow Publications 2014-09-01
• Series: Asian Pacific Journal of Reproduction
• Subjects: Apoptosis factor; Hormone; Synthetic dyes; Reproductive toxicology
• Online Access: http://www.sciencedirect.com/science/article/pii/S2305050014600233

Objective: To investigate whether orally exposure to rhodamine B could be changes the expression of Bax, Bcl-2 of the hypothalamic, and also levels of Follicle Stimulating Hormone (FSH) and Luteinizing Hormone (LH) in female rats. Methods: Twenty eight virgin female Wistar rats were divided into four groups, including control group, group exposed to dose of 4.5, 9 and 18 milligram/200 gram body weight (mg/200 g BW) of rhodamine B daily for 36 days. The hypothalamic expressions of Bax and Bcl-2 were examined immunohistochemically. The levels of serum FSH and LH were determined by the enzyme-linked immunosorbent assay (ELISA) technique. Results: The level of Bax was significantly higher in the rhodamine B treatment group compred to control group (P<0.05). Out of the 4.5, 9, and 18 mg/200 g BW doses of rhodamine B treatment, only the two highest doses significantly decreased the Bcl-2 levels compared to the control group (P<0.05). The serum FSH and LH levels were significantly lower in all dose's rhodamine B treatment groups compared with the control (P<0.05). Conclusion: In conclusion, rhodamine B increases hypothalamic cell apoptosis and disrupts hormonal balance in rats.