Distinguishing the parasitic wasp, Peristenus howardi, from some of its congeners using polymerase chain reaction and restriction endonuclease digestion
A molecular procedure incorporating polymerase chain reaction (PCR) of the COI gene and restriction endonuclease digestion of PCR products was used to distinguish Peristenus howardi (Hymenoptera: Braconidae) from four other Peristenus species. Non-solvent extraction of parasite DNA using a commercia...
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2004-07-01
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doaj-ed71f631c69345d693a05c21266b8d9e2020-11-24T21:32:00ZengOxford University PressJournal of Insect Science1536-24422004-07-01425Distinguishing the parasitic wasp, Peristenus howardi, from some of its congeners using polymerase chain reaction and restriction endonuclease digestionThomas M. MowryJames D. BarbourA molecular procedure incorporating polymerase chain reaction (PCR) of the COI gene and restriction endonuclease digestion of PCR products was used to distinguish Peristenus howardi (Hymenoptera: Braconidae) from four other Peristenus species. Non-solvent extraction of parasite DNA using a commercially available kit proved to be very effective in producing amplifiable template. Use of SfcI endonuclease produced restriction fragments with banding patterns in agarose gel electrophoresis that readily separated P. howardi, P. digoneutis, P. conradi, P. pallipes, and P. pseudopallipes.. However, while the restriction fragment banding patterns of both P. pallipes and P. pseudopallipes were easily distinguishable from the other Peristenus species, they could not be reliably separated from one another. This molecular procedure can be used in applied and ecological research to better understand the role of P. howardi in the Peristenus-Lygus parasite-host system within the Pacific Northwest. Consensus sequences of our amplimers for all five Peristenus spp. are deposited in GenBank under accession numbers AY626370, AY626371, AY626372, AY626373, and AY626374.http://www.insectscience.org/4.25/ |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Thomas M. Mowry James D. Barbour |
spellingShingle |
Thomas M. Mowry James D. Barbour Distinguishing the parasitic wasp, Peristenus howardi, from some of its congeners using polymerase chain reaction and restriction endonuclease digestion Journal of Insect Science |
author_facet |
Thomas M. Mowry James D. Barbour |
author_sort |
Thomas M. Mowry |
title |
Distinguishing the parasitic wasp, Peristenus howardi, from some of its congeners using polymerase chain reaction and restriction endonuclease digestion |
title_short |
Distinguishing the parasitic wasp, Peristenus howardi, from some of its congeners using polymerase chain reaction and restriction endonuclease digestion |
title_full |
Distinguishing the parasitic wasp, Peristenus howardi, from some of its congeners using polymerase chain reaction and restriction endonuclease digestion |
title_fullStr |
Distinguishing the parasitic wasp, Peristenus howardi, from some of its congeners using polymerase chain reaction and restriction endonuclease digestion |
title_full_unstemmed |
Distinguishing the parasitic wasp, Peristenus howardi, from some of its congeners using polymerase chain reaction and restriction endonuclease digestion |
title_sort |
distinguishing the parasitic wasp, peristenus howardi, from some of its congeners using polymerase chain reaction and restriction endonuclease digestion |
publisher |
Oxford University Press |
series |
Journal of Insect Science |
issn |
1536-2442 |
publishDate |
2004-07-01 |
description |
A molecular procedure incorporating polymerase chain reaction (PCR) of the COI gene and restriction endonuclease digestion of PCR products was used to distinguish Peristenus howardi (Hymenoptera: Braconidae) from four other Peristenus species. Non-solvent extraction of parasite DNA using a commercially available kit proved to be very effective in producing amplifiable template. Use of SfcI endonuclease produced restriction fragments with banding patterns in agarose gel electrophoresis that readily separated P. howardi, P. digoneutis, P. conradi, P. pallipes, and P. pseudopallipes.. However, while the restriction fragment banding patterns of both P. pallipes and P. pseudopallipes were easily distinguishable from the other Peristenus species, they could not be reliably separated from one another. This molecular procedure can be used in applied and ecological research to better understand the role of P. howardi in the Peristenus-Lygus parasite-host system within the Pacific Northwest. Consensus sequences of our amplimers for all five Peristenus spp. are deposited in GenBank under accession numbers AY626370, AY626371, AY626372, AY626373, and AY626374. |
url |
http://www.insectscience.org/4.25/ |
work_keys_str_mv |
AT thomasmmowry distinguishingtheparasiticwaspperistenushowardifromsomeofitscongenersusingpolymerasechainreactionandrestrictionendonucleasedigestion AT jamesdbarbour distinguishingtheparasiticwaspperistenushowardifromsomeofitscongenersusingpolymerasechainreactionandrestrictionendonucleasedigestion |
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