<i>ln vitro</i> isolation of <i>Ehrlichia ruminantium</i> from ovine blood into <i>lxodes scapularis</i> (lDE8) cell cultures

<i>ln vitro</i> isolation of <i>Ehrlichia ruminantium</i> from ovine blood into <i>lxodes scapularis</i> (lDE8) cell cultures

Four stocks of Ehrlichiar uminantium (Welgevonden, Ball3, Nonile and Blaauwkrans), the causative agent of heartwater in domestic ruminants, were isolated into lxodes capularis (lDE8) tick cells using the leukocyte fraction of the blood of infected sheep. Organisms of two of the E. ruminantrum stocks...

Full description

Bibliographic Details
Main Authors: E. Zweygarth, A. I. Josemans, H. C. Steyn
Format: Article
Language:English
Published: AOSIS 2008-08-01
Series:Onderstepoort Journal of Veterinary Research
Online Access:https://ojvr.org/index.php/ojvr/article/view/10
id doaj-ed51ad5240b64be898db9df5b0241f84
record_format Article
spelling doaj-ed51ad5240b64be898db9df5b0241f842020-11-24T23:04:30ZengAOSISOnderstepoort Journal of Veterinary Research0030-24652219-06352008-08-0175212112610.4102/ojvr.v75i2.105<i>ln vitro</i> isolation of <i>Ehrlichia ruminantium</i> from ovine blood into <i>lxodes scapularis</i> (lDE8) cell culturesE. ZweygarthA. I. JosemansH. C. SteynFour stocks of Ehrlichiar uminantium (Welgevonden, Ball3, Nonile and Blaauwkrans), the causative agent of heartwater in domestic ruminants, were isolated into lxodes capularis (lDE8) tick cells using the leukocyte fraction of the blood of infected sheep. Organisms of two of the E. ruminantrum stocks (Welgevonden and Blaauwkrans) propagated in IDEB cells were also successfully used to infect bovine endothelial cells. All stocks were successfully propagated in IDEB cells using Dulbecco's modified Eagle's medium nutrient mixture Ham F-12c ontaining 10% foetal bovine serum (FBS). The technique should be included in any attempt to isolate uncharacterized E. ruminantium stocks.https://ojvr.org/index.php/ojvr/article/view/10
collection DOAJ
language English
format Article
sources DOAJ
author E. Zweygarth
A. I. Josemans
H. C. Steyn
spellingShingle E. Zweygarth
A. I. Josemans
H. C. Steyn
<i>ln vitro</i> isolation of <i>Ehrlichia ruminantium</i> from ovine blood into <i>lxodes scapularis</i> (lDE8) cell cultures
Onderstepoort Journal of Veterinary Research
author_facet E. Zweygarth
A. I. Josemans
H. C. Steyn
author_sort E. Zweygarth
title <i>ln vitro</i> isolation of <i>Ehrlichia ruminantium</i> from ovine blood into <i>lxodes scapularis</i> (lDE8) cell cultures
title_short <i>ln vitro</i> isolation of <i>Ehrlichia ruminantium</i> from ovine blood into <i>lxodes scapularis</i> (lDE8) cell cultures
title_full <i>ln vitro</i> isolation of <i>Ehrlichia ruminantium</i> from ovine blood into <i>lxodes scapularis</i> (lDE8) cell cultures
title_fullStr <i>ln vitro</i> isolation of <i>Ehrlichia ruminantium</i> from ovine blood into <i>lxodes scapularis</i> (lDE8) cell cultures
title_full_unstemmed <i>ln vitro</i> isolation of <i>Ehrlichia ruminantium</i> from ovine blood into <i>lxodes scapularis</i> (lDE8) cell cultures
title_sort <i>ln vitro</i> isolation of <i>ehrlichia ruminantium</i> from ovine blood into <i>lxodes scapularis</i> (lde8) cell cultures
publisher AOSIS
series Onderstepoort Journal of Veterinary Research
issn 0030-2465
2219-0635
publishDate 2008-08-01
description Four stocks of Ehrlichiar uminantium (Welgevonden, Ball3, Nonile and Blaauwkrans), the causative agent of heartwater in domestic ruminants, were isolated into lxodes capularis (lDE8) tick cells using the leukocyte fraction of the blood of infected sheep. Organisms of two of the E. ruminantrum stocks (Welgevonden and Blaauwkrans) propagated in IDEB cells were also successfully used to infect bovine endothelial cells. All stocks were successfully propagated in IDEB cells using Dulbecco's modified Eagle's medium nutrient mixture Ham F-12c ontaining 10% foetal bovine serum (FBS). The technique should be included in any attempt to isolate uncharacterized E. ruminantium stocks.
url https://ojvr.org/index.php/ojvr/article/view/10
work_keys_str_mv AT ezweygarth ilnvitroiisolationofiehrlichiaruminantiumifromovinebloodintoilxodesscapularisilde8cellcultures
AT aijosemans ilnvitroiisolationofiehrlichiaruminantiumifromovinebloodintoilxodesscapularisilde8cellcultures
AT hcsteyn ilnvitroiisolationofiehrlichiaruminantiumifromovinebloodintoilxodesscapularisilde8cellcultures
_version_ 1725629912994283520

Similar Items