Large-scale 3D imaging of mouse cochlea using serial block-face scanning electron microscopy

Large-scale 3D imaging of mouse cochlea using serial block-face scanning electron microscopy

Summary: This protocol describes how to prepare intact mouse cochleae for serial block-face scanning electron microscopy (SBEM). The detailed workflow includes cochlea fixation, en bloc staining, resin embedding, X-ray microscopy-guided trimming and SBEM data acquisition. This protocol allows large-...

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Bibliographic Details
Main Authors: Yan Lu, Fangfang Wang, Haoyu Wang, Philipp Bastians, Yunfeng Hua
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Microscopy
Neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721002227

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