Large-scale 3D imaging of mouse cochlea using serial block-face scanning electron microscopy

Summary: This protocol describes how to prepare intact mouse cochleae for serial block-face scanning electron microscopy (SBEM). The detailed workflow includes cochlea fixation, en bloc staining, resin embedding, X-ray microscopy-guided trimming and SBEM data acquisition. This protocol allows large-...

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Main Authors:	Yan Lu, Fangfang Wang, Haoyu Wang, Philipp Bastians, Yunfeng Hua
Format:	Article
Language:	English
Published:	Elsevier 2021-06-01
Series:	STAR Protocols
Subjects:	Cell Biology Microscopy Neuroscience
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166721002227

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spelling	doaj-ed285b137979403285dbc87a080d36ac2021-06-21T04:25:30ZengElsevierSTAR Protocols2666-16672021-06-0122100515Large-scale 3D imaging of mouse cochlea using serial block-face scanning electron microscopyYan Lu0Fangfang Wang1Haoyu Wang2Philipp Bastians3Yunfeng Hua4Shanghai Institute of Precision Medicine, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Institute of Precision Medicine, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Corresponding authorShanghai Institute of Precision Medicine, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaZEISS Research Microscopy Solutions, Shanghai, ChinaShanghai Institute of Precision Medicine, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Corresponding authorSummary: This protocol describes how to prepare intact mouse cochleae for serial block-face scanning electron microscopy (SBEM). The detailed workflow includes cochlea fixation, en bloc staining, resin embedding, X-ray microscopy-guided trimming and SBEM data acquisition. This protocol allows large-scale, nanometer-resolution three-dimensional imaging of subcellular structures in a targeted tonotopic range of the cochlea and enables fast volumetric scan at submicron resolution using a compact X-ray microscope.For complete details on the use and execution of this protocol, please refer to Hua et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166721002227Cell BiologyMicroscopyNeuroscience
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Yan Lu Fangfang Wang Haoyu Wang Philipp Bastians Yunfeng Hua
spellingShingle	Yan Lu Fangfang Wang Haoyu Wang Philipp Bastians Yunfeng Hua Large-scale 3D imaging of mouse cochlea using serial block-face scanning electron microscopy STAR Protocols Cell Biology Microscopy Neuroscience
author_facet	Yan Lu Fangfang Wang Haoyu Wang Philipp Bastians Yunfeng Hua
author_sort	Yan Lu
title	Large-scale 3D imaging of mouse cochlea using serial block-face scanning electron microscopy
title_short	Large-scale 3D imaging of mouse cochlea using serial block-face scanning electron microscopy
title_full	Large-scale 3D imaging of mouse cochlea using serial block-face scanning electron microscopy
title_fullStr	Large-scale 3D imaging of mouse cochlea using serial block-face scanning electron microscopy
title_full_unstemmed	Large-scale 3D imaging of mouse cochlea using serial block-face scanning electron microscopy
title_sort	large-scale 3d imaging of mouse cochlea using serial block-face scanning electron microscopy
publisher	Elsevier
series	STAR Protocols
issn	2666-1667
publishDate	2021-06-01
description	Summary: This protocol describes how to prepare intact mouse cochleae for serial block-face scanning electron microscopy (SBEM). The detailed workflow includes cochlea fixation, en bloc staining, resin embedding, X-ray microscopy-guided trimming and SBEM data acquisition. This protocol allows large-scale, nanometer-resolution three-dimensional imaging of subcellular structures in a targeted tonotopic range of the cochlea and enables fast volumetric scan at submicron resolution using a compact X-ray microscope.For complete details on the use and execution of this protocol, please refer to Hua et al. (2021).
topic	Cell Biology Microscopy Neuroscience
url	http://www.sciencedirect.com/science/article/pii/S2666166721002227
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Large-scale 3D imaging of mouse cochlea using serial block-face scanning electron microscopy

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