Antibacterial effects Pulicaria gnaphalodes extract on some gram positive and negative bacteria

• Main Authors: Jamshidi-Moghaddam Y, Rajabizadeh A, Khazaeli P
• Format: Article
• Language: fas
• Published: Kashan University of Medical Sciences and Health Services 2020-12-01
• Series: Fiyz̤
• Subjects: p. gnaphalodes; plant extracts; anti-bacterial agents; gram-positive bacteria; gram-negative bacteria
• Online Access: http://feyz.kaums.ac.ir/article-1-4041-en.html

Background: The general tendency of the community to use herbal medicines is due to prove the destructive effects of chemical drugs and environmental pollution. This study aimed to investigate the Anti-bacterial properties of P. gnaphalodes extract on some gram-positive and negative bacteria. Materials and Methods: In this study, all of the organs of the P. gnaphalodes plant was dried in the shade in the summer of 2016 after collection. The Anti-bacterial effect of the plant was measured on Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Bacillus cereus to obtain the best extraction method. Next, 15 ml of Muller-Hinton-Agar medium was mixed with concentrations of 10 - 5.2 - 0.25 - 0.625 and 20 mg/ml of the extracts and 104 CFU /ml of the bacterium was inoculated and cultured after growing the colonies. In order to determine the MIC and MBC, the same volumes of 105 CFU/ ml were inoculated into Muller Hinton broth medium containing different concentrations of herbicide extracts. The culture medium was incubated for 24 hours at 37 ° C. Results: Concentration of 20 mg/ml had the most effect on inhibition of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Bacillus cereus bacteria, which was eliminated for 90, 68, 75 and 90% bacteria, respectively. MIC and MBC of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Bacillus cereus were obtained at 25, 50, 50, 70, 50, 80 and 30 and 50 mg/ml, respectively. Conclusion: The results showed that the extract of P. gnaphalodes has anti-bacterial effects on some gram positive and negative bacteria.