Expression of bladder α1-adrenoceptor subtype after relief of partial bladder outlet obstruction in a rat model

• Main Authors: Ji Yong Lee, Jong Mok Park, Yong Gil Na, Ki Hak Song, Jae Sung Lim, Seung Woo Yang, Gun Hwa Kim, Ju Hyun Shin
• Format: Article
• Language: English
• Published: Korean Urological Association 2020-05-01
• Series: Investigative and Clinical Urology
• Subjects: adrenergic alpha-1 receptor antagonists; lower urinary tract symptoms; urinary bladder neck obstruction; urinary bladder; overactive
• Online Access: https://www.icurology.org/Synapse/Data/PDFData/2020ICU/icu-61-297.pdf

Purpose: Many patients with benign prostatic hyperplasia require treatment for persistent storage symptoms, even when the obstruction is successfully relieved by surgery. Previous studies identified a characteristic increase in α1D-adrenoceptor levels in the bladder in a bladder outlet obstruction (BOO) model. Here, we investigated the expression of α1-adrenoceptor subtypes in the bladder after relief of partial BOO (pBOO) in a rat model. Materials and Methods: A total of 60 female Sprague–Dawley rats were randomly divided into three groups (sham-operated, pBOO, and pBOO relief groups), and the expression of α1-adrenoceptor subtypes in the urothelium and detrusor muscle tissues was examined by western blot. Results: The expression of the α1D-adrenoceptor was significantly higher in the urothelium and detrusor muscle tissue of the pBOO and pBOO relief groups than in the corresponding tissue of the sham-operated group. Additionally, the α1A-adrenoceptor was predominant in the sham-operated group but significantly decreased in the urothelium in the pBOO group. No significant differences were found in α1A-adrenoceptor levels in detrusor muscle or whole bladder. Conclusions: Our results showed that α1D-adrenoceptor levels were consistently increased with pBOO, even after relief, suggesting that the α1D-adrenoceptor might be a cause of persistent storage symptoms after relief of pBOO.