Monitoring Epithelial–Mesenchymal Transition of Pancreatic Cancer Cells via Investigation of Mitochondrial Dysfunction

Monitoring Epithelial–Mesenchymal Transition of Pancreatic Cancer Cells via Investigation of Mitochondrial Dysfunction

In this protocol, we introduced a method of measuring mitochondrial dysfunction to confirm the epithelial–mesenchymal transition (EMT) in pancreatic cancer cells under a hypoxic environment. There are many expertized and complicated methods to verify EMT. However, our methods have indicated that EMT...

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Bibliographic Details
Main Authors: Jae Jun Sim, Keun-Yeong Jeong
Format: Article
Language:English
Published: MDPI AG 2020-04-01
Series:Methods and Protocols
Subjects:
pancreatic cancer cell
hypoxia
ROS
mitochondria
membrane potential
epithelial–mesenchymal transition
Online Access:https://www.mdpi.com/2409-9279/3/2/32

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