Analysis of single nucleotide polymorphism (SNP) RS23472497 associated with canine atopic dermatitis by ACRS-PCR method

• Main Authors: Martina Miluchová, Michal Gábor, Anna Trakovická, Jana Hanusova, Radovan Kasarda
• Format: Article
• Language: English
• Published: Agroprint Timisoara 2014-05-01
• Series: Scientific Papers Animal Science and Biotechnologies
• Subjects: acrs-pcr, canine atopic dermatitis, dog, rs23472497, snp
• Online Access: http://spasb.ro/index.php/spasb/article/view/1759

The aim of the paper was to identify of the SNP rs23472497 associated with canine atopic dermatitis (cAD). cAD is a common inflammatory skin disease that is considered to be a naturally occurring, spontaneous model of human atopic dermatitis (eczema). The material involved 60 dogs from 6 different breeds. Canine genomic DNA was isolated from saliva by modified method with using DNAzol® and linear polyacrylamide (LPA) carrier and from blood by using commercial kit NucleospinBlood and used in order to estimate rs23472497 SNP genotypes by ACRS-PCR method. The PCR products were digested with NlaIII restriction enzyme. In the population of Czech Pointer and Slovak Wirehaired Pointer we detected all genotypes AA, AG and GG with frequency 0.0732, 0.5122 and 0.4146 for Czech Pointer and 0.1818, 0.5455 and 0.2727 for Slovak Wirehaired Pointer. In Border Collie was observed heterozygote genotype AG and homozygote genotype GG with frequency 0.6667 and 0.3333, subsequently. In German Wirehaired Pointer, Australian Shepherd dog and American Staffordshire terrier we detected only genotype AG with frequency 1. The A allele was distributed with an allele frequency ranging from 0.3293 to 0.5. The G allele was distributed with an allele frequency ranging from 0.5 to 0.6707.