Improving redox sensitivity of roGFP1 by incorporation of selenocysteine at position 147

Improving redox sensitivity of roGFP1 by incorporation of selenocysteine at position 147

Abstract Objective Redox-sensitive green fluorescent protein (roGFP) is a genetically-encoded redox-sensitive protein used to detect cellular oxidative stress associated with reactive oxygen species production. Here we replaced the cysteine at position 147 of roGFP1 (variant of roGFP) with selenocys...

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Bibliographic Details
Main Authors: Katherine R. Stanford, Joanne M. Ajmo, Parmvir K. Bahia, Stephen H. Hadley, Thomas E. Taylor-Clark
Format: Article
Language:English
Published: BMC 2018-11-01
Series:BMC Research Notes
Subjects:
Green fluorescent protein
Reporter
Redox-sensing
Selenocysteine
Reactive oxygen species
Online Access:http://link.springer.com/article/10.1186/s13104-018-3929-x

Internet

http://link.springer.com/article/10.1186/s13104-018-3929-x

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