Production of Single-Chain Fv Antibodies Specific for GA-Pyridine, an Advanced Glycation End-Product (AGE), with Reduced Inter-Domain Motion

Production of Single-Chain Fv Antibodies Specific for GA-Pyridine, an Advanced Glycation End-Product (AGE), with Reduced Inter-Domain Motion

Due to their lower production cost compared with monoclonal antibodies, single-chain variable fragments (scFvs) have potential for use in several applications, such as for diagnosis and treatment of a range of diseases, and as sensor elements. However, the usefulness of scFvs is limited by inhomogen...

Full description

Bibliographic Details
Main Authors: Natsuki Fukuda, Kentaro Noi, Lidong Weng, Yoshihiro Kobashigawa, Hiromi Miyazaki, Yukari Wakeyama, Michiyo Takaki, Yusuke Nakahara, Yuka Tatsuno, Makiyo Uchida-Kamekura, Yoshiaki Suwa, Takashi Sato, Naoki Ichikawa-Tomikawa, Motoyoshi Nomizu, Yukio Fujiwara, Fumina Ohsaka, Takashi Saitoh, Katsumi Maenaka, Hiroyuki Kumeta, Shoko Shinya, Chojiro Kojima, Teru Ogura, Hiroshi Morioka
Format: Article
Language:English
Published: MDPI AG 2017-10-01
Series:Molecules
Subjects:
GA-pyridine
single-chain variable fragment
phage display
isothermal titration calorimetry
differential scanning calorimetry
small-angle X-ray scattering
high-speed atomic force microscopy
NMR analysis
inter-domain motion
Online Access:https://www.mdpi.com/1420-3049/22/10/1695

Internet

https://www.mdpi.com/1420-3049/22/10/1695

Similar Items