Relationship between hepatitis B virus DNA and Th1/Th2 in chronic hepatitis B patients

• Main Authors: Feng-Xia Chang, Juan Hao, Mao-Cuo Peng, Ming Ma, Li-Yuan Luo
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2016-08-01
• Series: Journal of Hainan Medical University
• Subjects: Chronic hepatitis b; HBV DNA; Th1/Th2; Relationship
• Online Access: http://www.hnykdxxb.com/PDF/201615/11.pdf

Objective: To investigate the relationship between the hepatitis B virus (HBV) DNA level of chronic hepatitis B patients and Th1/Th2. Methods: Based on the level of HBV DNA, 135 CHB patients were divided into two groups, high level group (the level of HBV DNA 1×106- 1×107 copies/mL), and low level group (the level of HBV DNA 1×104-1×105 copies/mL), with 56 and 79 patients, respectively. In addition, 45 healthy volunteers were recruited as control group. Furthermore, index of liver function were compared between these three groups, containing glutamic-pyruvic transaminase (ALT), glutamic oxalacetictransaminase (AST), alkaline phosphatase (ALP), markers of HBV, including HBsAg, HBeAg, Anti-HBe, Th1 and Th2 by using flow cytometry. Results: ALT, AST and ALP were significantly different between two groups (P<0.05). ALT, AST and ALP of high level group were significantly higher than control group and low level group, ALT, AST and ALP of low level group were significantly higher than control group (P<0.05). HBsAg, HBeAg of high level group were significantly higher than low level group, however, anti-HBe was lower in high level group than low level group (P<0.05). Th1, Th1/Th2 of high level group were significantly lower than low level group (P<0.05). But there was no significantly difference of Th2 level between two groups (P>0.05). There was a negative relationship between HBV DNA level and Th1, Th1/ Th2 r=-0.391, -0.228, P<0.05), no relevance with Th2 (r=0.029, P>0.05).Conclusions: Th1/ Th2 is imbalanced in chronic hepatitis B patients, displaying low level of Th1/Th2, which is related to level of virus copies.