Phosphorus compounds, proteins, nuclease and acid phosphatase activities in isolated spinach chloroplasts

This paper deals with attempts to elaborate a simple method of spinach chloroplast isolation ensuring a high proportion of intact chloroplasts. We obtained 3 preparations of isolated chloroplasts. Several preliminary analyses of the obtained chloroplast fraction were also performed. Phosphorus compo...

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Main Authors: E. Mikulska, W. Maciejewska-Potapczyk, I. Konopska, B. Damsz
Format: Article
Language:English
Published: Polish Botanical Society 2015-01-01
Series:Acta Societatis Botanicorum Poloniae
Online Access:https://pbsociety.org.pl/journals/index.php/asbp/article/view/5222
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spelling doaj-ec022c051d6747879f45091cde3ffdb22020-11-25T03:16:58ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802015-01-0141221722210.5586/asbp.1972.0164424Phosphorus compounds, proteins, nuclease and acid phosphatase activities in isolated spinach chloroplastsE. Mikulska0W. Maciejewska-Potapczyk1I. Konopska2B. Damsz3University of ŁódźUniversity of ŁódźUniversity of ŁódźUniversity of ŁódźThis paper deals with attempts to elaborate a simple method of spinach chloroplast isolation ensuring a high proportion of intact chloroplasts. We obtained 3 preparations of isolated chloroplasts. Several preliminary analyses of the obtained chloroplast fraction were also performed. Phosphorus compounds, total protein and the enzyme activities of RNase, DNase and GPase were determined. We found: 0,36-0,59% of RNA, 0,19-0,24% of DNA, 2,1-2,9% of phospholipids and 26-28% of protein. RNase activity was very high.https://pbsociety.org.pl/journals/index.php/asbp/article/view/5222
collection DOAJ
language English
format Article
sources DOAJ
author E. Mikulska
W. Maciejewska-Potapczyk
I. Konopska
B. Damsz
spellingShingle E. Mikulska
W. Maciejewska-Potapczyk
I. Konopska
B. Damsz
Phosphorus compounds, proteins, nuclease and acid phosphatase activities in isolated spinach chloroplasts
Acta Societatis Botanicorum Poloniae
author_facet E. Mikulska
W. Maciejewska-Potapczyk
I. Konopska
B. Damsz
author_sort E. Mikulska
title Phosphorus compounds, proteins, nuclease and acid phosphatase activities in isolated spinach chloroplasts
title_short Phosphorus compounds, proteins, nuclease and acid phosphatase activities in isolated spinach chloroplasts
title_full Phosphorus compounds, proteins, nuclease and acid phosphatase activities in isolated spinach chloroplasts
title_fullStr Phosphorus compounds, proteins, nuclease and acid phosphatase activities in isolated spinach chloroplasts
title_full_unstemmed Phosphorus compounds, proteins, nuclease and acid phosphatase activities in isolated spinach chloroplasts
title_sort phosphorus compounds, proteins, nuclease and acid phosphatase activities in isolated spinach chloroplasts
publisher Polish Botanical Society
series Acta Societatis Botanicorum Poloniae
issn 2083-9480
publishDate 2015-01-01
description This paper deals with attempts to elaborate a simple method of spinach chloroplast isolation ensuring a high proportion of intact chloroplasts. We obtained 3 preparations of isolated chloroplasts. Several preliminary analyses of the obtained chloroplast fraction were also performed. Phosphorus compounds, total protein and the enzyme activities of RNase, DNase and GPase were determined. We found: 0,36-0,59% of RNA, 0,19-0,24% of DNA, 2,1-2,9% of phospholipids and 26-28% of protein. RNase activity was very high.
url https://pbsociety.org.pl/journals/index.php/asbp/article/view/5222
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AT ikonopska phosphoruscompoundsproteinsnucleaseandacidphosphataseactivitiesinisolatedspinachchloroplasts
AT bdamsz phosphoruscompoundsproteinsnucleaseandacidphosphataseactivitiesinisolatedspinachchloroplasts
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