Phosphorus compounds, proteins, nuclease and acid phosphatase activities in isolated spinach chloroplasts
This paper deals with attempts to elaborate a simple method of spinach chloroplast isolation ensuring a high proportion of intact chloroplasts. We obtained 3 preparations of isolated chloroplasts. Several preliminary analyses of the obtained chloroplast fraction were also performed. Phosphorus compo...
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Polish Botanical Society
2015-01-01
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Series: | Acta Societatis Botanicorum Poloniae |
Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/5222 |
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doaj-ec022c051d6747879f45091cde3ffdb22020-11-25T03:16:58ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802015-01-0141221722210.5586/asbp.1972.0164424Phosphorus compounds, proteins, nuclease and acid phosphatase activities in isolated spinach chloroplastsE. Mikulska0W. Maciejewska-Potapczyk1I. Konopska2B. Damsz3University of ŁódźUniversity of ŁódźUniversity of ŁódźUniversity of ŁódźThis paper deals with attempts to elaborate a simple method of spinach chloroplast isolation ensuring a high proportion of intact chloroplasts. We obtained 3 preparations of isolated chloroplasts. Several preliminary analyses of the obtained chloroplast fraction were also performed. Phosphorus compounds, total protein and the enzyme activities of RNase, DNase and GPase were determined. We found: 0,36-0,59% of RNA, 0,19-0,24% of DNA, 2,1-2,9% of phospholipids and 26-28% of protein. RNase activity was very high.https://pbsociety.org.pl/journals/index.php/asbp/article/view/5222 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
E. Mikulska W. Maciejewska-Potapczyk I. Konopska B. Damsz |
spellingShingle |
E. Mikulska W. Maciejewska-Potapczyk I. Konopska B. Damsz Phosphorus compounds, proteins, nuclease and acid phosphatase activities in isolated spinach chloroplasts Acta Societatis Botanicorum Poloniae |
author_facet |
E. Mikulska W. Maciejewska-Potapczyk I. Konopska B. Damsz |
author_sort |
E. Mikulska |
title |
Phosphorus compounds, proteins, nuclease and acid phosphatase activities in isolated spinach chloroplasts |
title_short |
Phosphorus compounds, proteins, nuclease and acid phosphatase activities in isolated spinach chloroplasts |
title_full |
Phosphorus compounds, proteins, nuclease and acid phosphatase activities in isolated spinach chloroplasts |
title_fullStr |
Phosphorus compounds, proteins, nuclease and acid phosphatase activities in isolated spinach chloroplasts |
title_full_unstemmed |
Phosphorus compounds, proteins, nuclease and acid phosphatase activities in isolated spinach chloroplasts |
title_sort |
phosphorus compounds, proteins, nuclease and acid phosphatase activities in isolated spinach chloroplasts |
publisher |
Polish Botanical Society |
series |
Acta Societatis Botanicorum Poloniae |
issn |
2083-9480 |
publishDate |
2015-01-01 |
description |
This paper deals with attempts to elaborate a simple method of spinach chloroplast isolation ensuring a high proportion of intact chloroplasts. We obtained 3 preparations of isolated chloroplasts. Several preliminary analyses of the obtained chloroplast fraction were also performed. Phosphorus compounds, total protein and the enzyme activities of RNase, DNase and GPase were determined. We found: 0,36-0,59% of RNA, 0,19-0,24% of DNA, 2,1-2,9% of phospholipids and 26-28% of protein. RNase activity was very high. |
url |
https://pbsociety.org.pl/journals/index.php/asbp/article/view/5222 |
work_keys_str_mv |
AT emikulska phosphoruscompoundsproteinsnucleaseandacidphosphataseactivitiesinisolatedspinachchloroplasts AT wmaciejewskapotapczyk phosphoruscompoundsproteinsnucleaseandacidphosphataseactivitiesinisolatedspinachchloroplasts AT ikonopska phosphoruscompoundsproteinsnucleaseandacidphosphataseactivitiesinisolatedspinachchloroplasts AT bdamsz phosphoruscompoundsproteinsnucleaseandacidphosphataseactivitiesinisolatedspinachchloroplasts |
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