CTLA-4 Expression and Polymorphisms in Lung Tissue of Patients with Diagnosed Non-Small-Cell Lung Cancer

Cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4) is a potent immunoregulatory molecule that downregulates T-cell activation and thus influences the antitumor immune response. CTLA-4 polymorphisms are associated with various cancers, and CTLA-4 mRNA/protein increased expression is found in severa...

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Main Authors: Adam Antczak, Dorota Pastuszak-Lewandoska, Paweł Górski, Daria Domańska, Monika Migdalska-Sęk, Karolina Czarnecka, Ewa Nawrot, Jacek Kordiak, Ewa Brzeziańska
Format: Article
Language:English
Published: Hindawi Limited 2013-01-01
Series:BioMed Research International
Online Access:http://dx.doi.org/10.1155/2013/576486
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spelling doaj-ec0065ee770e4c43ac7877079c48595e2020-11-24T22:19:35ZengHindawi LimitedBioMed Research International2314-61332314-61412013-01-01201310.1155/2013/576486576486CTLA-4 Expression and Polymorphisms in Lung Tissue of Patients with Diagnosed Non-Small-Cell Lung CancerAdam Antczak0Dorota Pastuszak-Lewandoska1Paweł Górski2Daria Domańska3Monika Migdalska-Sęk4Karolina Czarnecka5Ewa Nawrot6Jacek Kordiak7Ewa Brzeziańska8Department of General and Oncological Pulmonology, 1st Chair of Internal Diseases, Medical University of Lodz, Kopcińskiego 22, 90-153 Łódź, PolandDepartment of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, Pomorska 251, 92-213 Łódź, PolandDepartment of Pneumology and Allergology, 1st Chair of Internal Diseases, Medical University of Lodz, Kopcińskiego 22, 90-153 Łódź, PolandDepartment of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, Pomorska 251, 92-213 Łódź, PolandDepartment of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, Pomorska 251, 92-213 Łódź, PolandDepartment of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, Pomorska 251, 92-213 Łódź, PolandDepartment of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, Pomorska 251, 92-213 Łódź, PolandDepartment of Chest Surgery, General and Oncological Surgery University Hospital No. 2, Medical University of Lodz, Żeromskiego 113, 90-710 Łódź, PolandDepartment of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, Pomorska 251, 92-213 Łódź, PolandCytotoxic T-lymphocyte-associated antigen-4 (CTLA-4) is a potent immunoregulatory molecule that downregulates T-cell activation and thus influences the antitumor immune response. CTLA-4 polymorphisms are associated with various cancers, and CTLA-4 mRNA/protein increased expression is found in several tumor types. However, most of the studies are based on peripheral blood mononuclear cells, and much less is known about the relationship between CTLA-4 expression, especially gene expression, and its polymorphic variants in cancer tissue. In our study we assessed the distribution of CTLA-4 two polymorphisms (+49A/G and −318C/T), using TaqMan probes (rs231775 and rs5742909, resp.), and CTLA-4 gene expression in real-time PCR assay in non-small-cell lung cancer (NSCLC) tissue samples. The increased CTLA-4 expression was observed in the majority of NSCLC patients, and it was significantly correlated with TT genotype (−318C/T) and with tumor size (T2 versus T3 + T4). The presence of G allele and GG genotype in cancer tissue (+49A/G) was significantly associated with the increased NSCLC risk. Additionally, we compared genotype distributions in the corresponding tumor and blood samples and found statistically significant differences. The shift from one genotype in the blood to another in the tumor may confirm the complexity of gene functionality in cancer tissue.http://dx.doi.org/10.1155/2013/576486
collection DOAJ
language English
format Article
sources DOAJ
author Adam Antczak
Dorota Pastuszak-Lewandoska
Paweł Górski
Daria Domańska
Monika Migdalska-Sęk
Karolina Czarnecka
Ewa Nawrot
Jacek Kordiak
Ewa Brzeziańska
spellingShingle Adam Antczak
Dorota Pastuszak-Lewandoska
Paweł Górski
Daria Domańska
Monika Migdalska-Sęk
Karolina Czarnecka
Ewa Nawrot
Jacek Kordiak
Ewa Brzeziańska
CTLA-4 Expression and Polymorphisms in Lung Tissue of Patients with Diagnosed Non-Small-Cell Lung Cancer
BioMed Research International
author_facet Adam Antczak
Dorota Pastuszak-Lewandoska
Paweł Górski
Daria Domańska
Monika Migdalska-Sęk
Karolina Czarnecka
Ewa Nawrot
Jacek Kordiak
Ewa Brzeziańska
author_sort Adam Antczak
title CTLA-4 Expression and Polymorphisms in Lung Tissue of Patients with Diagnosed Non-Small-Cell Lung Cancer
title_short CTLA-4 Expression and Polymorphisms in Lung Tissue of Patients with Diagnosed Non-Small-Cell Lung Cancer
title_full CTLA-4 Expression and Polymorphisms in Lung Tissue of Patients with Diagnosed Non-Small-Cell Lung Cancer
title_fullStr CTLA-4 Expression and Polymorphisms in Lung Tissue of Patients with Diagnosed Non-Small-Cell Lung Cancer
title_full_unstemmed CTLA-4 Expression and Polymorphisms in Lung Tissue of Patients with Diagnosed Non-Small-Cell Lung Cancer
title_sort ctla-4 expression and polymorphisms in lung tissue of patients with diagnosed non-small-cell lung cancer
publisher Hindawi Limited
series BioMed Research International
issn 2314-6133
2314-6141
publishDate 2013-01-01
description Cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4) is a potent immunoregulatory molecule that downregulates T-cell activation and thus influences the antitumor immune response. CTLA-4 polymorphisms are associated with various cancers, and CTLA-4 mRNA/protein increased expression is found in several tumor types. However, most of the studies are based on peripheral blood mononuclear cells, and much less is known about the relationship between CTLA-4 expression, especially gene expression, and its polymorphic variants in cancer tissue. In our study we assessed the distribution of CTLA-4 two polymorphisms (+49A/G and −318C/T), using TaqMan probes (rs231775 and rs5742909, resp.), and CTLA-4 gene expression in real-time PCR assay in non-small-cell lung cancer (NSCLC) tissue samples. The increased CTLA-4 expression was observed in the majority of NSCLC patients, and it was significantly correlated with TT genotype (−318C/T) and with tumor size (T2 versus T3 + T4). The presence of G allele and GG genotype in cancer tissue (+49A/G) was significantly associated with the increased NSCLC risk. Additionally, we compared genotype distributions in the corresponding tumor and blood samples and found statistically significant differences. The shift from one genotype in the blood to another in the tumor may confirm the complexity of gene functionality in cancer tissue.
url http://dx.doi.org/10.1155/2013/576486
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