Potensi Ochrobactrum intermedium - C939A31, Klebsiella oxytoca - C939A32, Bacillus subtilis- I308A32 Asal Tanaman Kopi untuk Mengendalikan Nematoda Luka Akar Pratylenchus coffeae

• Main Authors: Dwi Halimah, Abdul Munif, Giyanto Giyanto
• Format: Article
• Language: Indonesian
• Published: Perhimpunan Fitopatologi Indonesia 2016-05-01
• Series: Jurnal Fitopatologi Indonesia
• Subjects: endophityc bacteria, haemolysis test, hypersensitive reaction, pathogenicity test
• Online Access: http://journal.ipb.ac.id/index.php/jfiti/article/view/12044
• ISSN: 0215-7950; 2339-2479

Infection of root lession nematode, <em>Pratylenchus coffeae</em>, becomes an important factor causing yield loss in coffee production in Indonesia. Biological control of <em>P. coffeae </em>needs to be developed to meet the requirement of environmentally save crop production.  The research was conducted to evaluate 3 selected endophytic bacteria isolates, <em>Ochrobactrum intermedium </em>DSQ5, <em>Klebsiella oxytoca </em>NGB-FR 50, and <em>Bacillus  subtilis </em>AIMST 10.T18.1, from coffee in controlling <em>P. coffeae</em>. Research methods involved pathogenicity test based on hypersensitive reaction and haemolysis test, and evaluation of their ability to suppress <em>P. coffeae in vitro </em>and <em>in planta</em>. The hypersensitive and haemolysis reactions indicated that these 3 isolates showed negative result both to plant and human. <em>In vitro</em> assays showed that two isolates, <em>K. oxytoca </em>- C939A32 and <em>O. intermedium - </em>C939A3, could suppress <em>P. coffeae</em> populations by 66.7% and 100%. Those results correlated positively with <em>in planta</em> assay’s result.