Transforming Growth Factor-alpha Induce Synthesis of alpha-1-Antitryspin in Human Hepatoma Cell Line (HepG2)

• Main Author: Mohammad Taghi Goodarzi
• Format: Article
• Language: fas
• Published: Hamadan University of Medical Sciences 1999-03-01
• Series: پزشکی بالینی ابن سینا
• Subjects: acute phase protein / alpha – 1 – antitrypsin / cytokines / transforming / growth factor alpha
• Online Access: http://sjh.umsha.ac.ir/article-1-973-en.html

Cytokines protein or glycoproteins synthesized by a wide range of cell types including lymphocytes, macrophages, platelets, and monocytes. Cytokines induse synthesis of acute phase protein in hepatocytes. So far eight cytokines has been shown can induce acute phase protein synthesis in hepatocyte and hepatoma cell culture. There are evidences that transforming growth factor-alpha (TGF-a) secrets by tumor cells. In the other hand conditioned medium from ovarian cancer cells can stimulate API synthesis in hepatoma cell line. To show the effect of TGA-a on API synthesis in human hepatoma cell line HepG2, two different concentration of TGF-a were added to culture media of HepG2 cells. API concentration culture media was measured using  rocket immunoassay. The results showed TGF-a in 5 ng/ml increased synthesis of API abut 29%. In higher concentration, TGF-a showed  lower effect. It can be suggested that TGF-a plays role in preventing the effect of proteolytic enzymes by inducing synthesis of anti-proteases.