Molecular analysis of various classes of integrons in the clinical Pseudomonas aeruginosa isolates by Multiplex-PCR

• Main Authors: Atousa Rashidi, Gholamali Moradli
• Format: Article
• Language: fas
• Published: Fasa University of Medical Sciences 2017-09-01
• Series: Journal of Fasa University of Medical Sciences
• Subjects: Class I; II; III Integron genes; Pseudomonas aeruginosa; Multiplex-PCR.
• Online Access: http://journal.fums.ac.ir/browse.php?a_code=A-10-839-2&slc_lang=en&sid=1

Background & Objective: The presence of classes I, II and III Integrons in multidrug-resistant Pseudomonas aeruginosa strains, has created new concerns in clinics. This study aimed to investigate the prevalence of class I, II and III Integron in clinical Pseudomonas aeruginosa isolates. Materials & Methods: In the cross-sectional study, 60 isolates of P. aeruginosa were obtained from the microbial-Laboratory of Rasool Akram Hospital in Tehran. Antibiotic susceptibility test was performed by the agar diffusion method based on CLSI guidelines on the Mueller Hinton agar medium. The genomic DNA was extracted using DNA extraction kit and M-PCR was performed for amplification of intI, intII and intIII genes. Results: The highest resistance rate was in the strains that harbored integron, so that 96.6% of strains were resistant to amoxicillin. The results showed that all 60 strains had resistance (100%) to amikacin. The highest frequency was related to intI (40%) and the lowest frequency was related to intIII genes (16.6%). Conclusion: Considering the high prevalence of class I Integron in all Pseudomonas aeruginosa-resistant isolates and its relation with different drug resistance patterns, the right solution to infection control and treatment in hospitals is essential to prevent further spread of it.