Approaches to gene pool conservation of medicinal plant Oxytropis lanata (Pall.) DC. (Fabaceae)
In order to preserve the gene pool of medicinal plant Oxytropis lanata (Pall.) DC. we analyzed allozyme polymorphism and identified reliable and informative marker enzyme systems of this species; also we studied the response of seeds to deep freezing in liquid nitrogen (–196 ºС). Population has an a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Altay State University
2015-05-01
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| Series: | Turczaninowia |
| Online Access: | http://journal.asu.ru/index.php/tur/article/view/748 |
| Summary: | In order to preserve the gene pool of medicinal plant Oxytropis lanata (Pall.) DC. we analyzed allozyme polymorphism and identified reliable and informative marker enzyme systems of this species; also we studied the response of seeds to deep freezing in liquid nitrogen (–196 ºС). Population has an average level of polymorphism (P95 = 41,2 %, P99 = 52,9 %, A = 1,58, Ho = 0,158, He = 0,171) in general typical for herbaceous legumes, and can serve as a source of material for gene pool conservation of the species. Deep freezing has not led to the death of the seeds; it was marked stimulatory effect of ultralow temperatures, expressed as an acceleration of germination and sharp increase of germinability (98,6 ± 2,3 %) compared to the control (12,0 ± 3,5 %) that is associated with overcoming physical dormancy. There were no abnormalities in the development of seedlings from seeds passed cryopreservation. |
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| ISSN: | 1560-7259 1560-7267 |