QS8: Enrichment of Nanofiber Hydrogel Composite with Fractionated Fat Promotes Pro-regenerative Macrophage Polarization and Angiogenesis for Soft Tissue Engineering
Purpose: Fractionated fat (FF) has been shown to promote dermal regeneration; however, the use of fat grafting for reconstruction of soft tissue defects is limited due to volume loss over time. We have developed a novel approach for engineering of vascularized soft tissue using an injectable nanofib...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer
2021-07-01
|
| Series: | Plastic and Reconstructive Surgery, Global Open |
| Online Access: | http://journals.lww.com/prsgo/fulltext/10.1097/01.GOX.0000769980.35543.2a |
| id |
doaj-eb774fa248704a04ab21a6b79d328f2a |
|---|---|
| record_format |
Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Dominic Henn, MD Katharina S. Fischer, MD Kellen Chen, PhD Autumn H. Greco, BS Russell A. Martin, PhD Dharshan Sivaraj, BS Artem A. Trotsyuk, BS Hai-Quan Mao, PhD Sashank K. Reddy, MD, PhD Ulrich Kneser, MD Geoffrey C. Gurtner, MD Volker J. Schmidt, MD Justin M. Sacks, MD |
| spellingShingle |
Dominic Henn, MD Katharina S. Fischer, MD Kellen Chen, PhD Autumn H. Greco, BS Russell A. Martin, PhD Dharshan Sivaraj, BS Artem A. Trotsyuk, BS Hai-Quan Mao, PhD Sashank K. Reddy, MD, PhD Ulrich Kneser, MD Geoffrey C. Gurtner, MD Volker J. Schmidt, MD Justin M. Sacks, MD QS8: Enrichment of Nanofiber Hydrogel Composite with Fractionated Fat Promotes Pro-regenerative Macrophage Polarization and Angiogenesis for Soft Tissue Engineering Plastic and Reconstructive Surgery, Global Open |
| author_facet |
Dominic Henn, MD Katharina S. Fischer, MD Kellen Chen, PhD Autumn H. Greco, BS Russell A. Martin, PhD Dharshan Sivaraj, BS Artem A. Trotsyuk, BS Hai-Quan Mao, PhD Sashank K. Reddy, MD, PhD Ulrich Kneser, MD Geoffrey C. Gurtner, MD Volker J. Schmidt, MD Justin M. Sacks, MD |
| author_sort |
Dominic Henn, MD |
| title |
QS8: Enrichment of Nanofiber Hydrogel Composite with Fractionated Fat Promotes Pro-regenerative Macrophage Polarization and Angiogenesis for Soft Tissue Engineering |
| title_short |
QS8: Enrichment of Nanofiber Hydrogel Composite with Fractionated Fat Promotes Pro-regenerative Macrophage Polarization and Angiogenesis for Soft Tissue Engineering |
| title_full |
QS8: Enrichment of Nanofiber Hydrogel Composite with Fractionated Fat Promotes Pro-regenerative Macrophage Polarization and Angiogenesis for Soft Tissue Engineering |
| title_fullStr |
QS8: Enrichment of Nanofiber Hydrogel Composite with Fractionated Fat Promotes Pro-regenerative Macrophage Polarization and Angiogenesis for Soft Tissue Engineering |
| title_full_unstemmed |
QS8: Enrichment of Nanofiber Hydrogel Composite with Fractionated Fat Promotes Pro-regenerative Macrophage Polarization and Angiogenesis for Soft Tissue Engineering |
| title_sort |
qs8: enrichment of nanofiber hydrogel composite with fractionated fat promotes pro-regenerative macrophage polarization and angiogenesis for soft tissue engineering |
| publisher |
Wolters Kluwer |
| series |
Plastic and Reconstructive Surgery, Global Open |
| issn |
2169-7574 |
| publishDate |
2021-07-01 |
| description |
Purpose: Fractionated fat (FF) has been shown to promote dermal regeneration; however, the use of fat grafting for reconstruction of soft tissue defects is limited due to volume loss over time. We have developed a novel approach for engineering of vascularized soft tissue using an injectable nanofiber hydrogel (NHC) enriched with FF.
Methods: FF was generated by emulsification of groin fat pads from rats and mixed in a 3:1 ratio with NHC (NHC-FF). NHC-FF or NHC alone were placed into isolation chambers together with arteriovenous (AV) loops, which were subcutaneously implanted into the groin of rats (n=8 per group). After 21 days, animals were euthanized, systemically perfused with ink, and tissue was explanted for histological analysis. Immunofluorescent staining (IF) and confocal laser scanning microscopy were used to quantify CD34+ progenitor cells and macrophage subpopulations.
Results: NHC-FF tissue maintained its shape without shrinking and showed a significantly stronger functional neovascularization compared to NHC alone at 21 days after implantation (mean vessel count: 833.5 ± 206.1 vs. 296.5 ± 114.1, p = 0.04). Tissue remodeling and cellular infiltration were greater in NHC-FF (mean cell count: 49,707 ± 18,491 vs. 9,263 ± 3,790, p = 0.005) with a significantly higher amount of progenitor cells and regenerative CD163+ macrophages compared to NHC alone.
Conclusion: FF-enriched NHC transforms into highly vascularized soft tissue over 21 days without signs of shrinking and promotes macrophage polarization toward regenerative phenotypes. Enrichment of injectable NHC with FF represents a promising approach for durable reconstruction of soft tissue defects. |
| url |
http://journals.lww.com/prsgo/fulltext/10.1097/01.GOX.0000769980.35543.2a |
| work_keys_str_mv |
AT dominichennmd qs8enrichmentofnanofiberhydrogelcompositewithfractionatedfatpromotesproregenerativemacrophagepolarizationandangiogenesisforsofttissueengineering AT katharinasfischermd qs8enrichmentofnanofiberhydrogelcompositewithfractionatedfatpromotesproregenerativemacrophagepolarizationandangiogenesisforsofttissueengineering AT kellenchenphd qs8enrichmentofnanofiberhydrogelcompositewithfractionatedfatpromotesproregenerativemacrophagepolarizationandangiogenesisforsofttissueengineering AT autumnhgrecobs qs8enrichmentofnanofiberhydrogelcompositewithfractionatedfatpromotesproregenerativemacrophagepolarizationandangiogenesisforsofttissueengineering AT russellamartinphd qs8enrichmentofnanofiberhydrogelcompositewithfractionatedfatpromotesproregenerativemacrophagepolarizationandangiogenesisforsofttissueengineering AT dharshansivarajbs qs8enrichmentofnanofiberhydrogelcompositewithfractionatedfatpromotesproregenerativemacrophagepolarizationandangiogenesisforsofttissueengineering AT artematrotsyukbs qs8enrichmentofnanofiberhydrogelcompositewithfractionatedfatpromotesproregenerativemacrophagepolarizationandangiogenesisforsofttissueengineering AT haiquanmaophd qs8enrichmentofnanofiberhydrogelcompositewithfractionatedfatpromotesproregenerativemacrophagepolarizationandangiogenesisforsofttissueengineering AT sashankkreddymdphd qs8enrichmentofnanofiberhydrogelcompositewithfractionatedfatpromotesproregenerativemacrophagepolarizationandangiogenesisforsofttissueengineering AT ulrichknesermd qs8enrichmentofnanofiberhydrogelcompositewithfractionatedfatpromotesproregenerativemacrophagepolarizationandangiogenesisforsofttissueengineering AT geoffreycgurtnermd qs8enrichmentofnanofiberhydrogelcompositewithfractionatedfatpromotesproregenerativemacrophagepolarizationandangiogenesisforsofttissueengineering AT volkerjschmidtmd qs8enrichmentofnanofiberhydrogelcompositewithfractionatedfatpromotesproregenerativemacrophagepolarizationandangiogenesisforsofttissueengineering AT justinmsacksmd qs8enrichmentofnanofiberhydrogelcompositewithfractionatedfatpromotesproregenerativemacrophagepolarizationandangiogenesisforsofttissueengineering |
| _version_ |
1721282260670349312 |
| spelling |
doaj-eb774fa248704a04ab21a6b79d328f2a2021-07-26T05:34:24ZengWolters KluwerPlastic and Reconstructive Surgery, Global Open2169-75742021-07-0197S101110.1097/01.GOX.0000769980.35543.2a202107001-00015QS8: Enrichment of Nanofiber Hydrogel Composite with Fractionated Fat Promotes Pro-regenerative Macrophage Polarization and Angiogenesis for Soft Tissue EngineeringDominic Henn, MD0Katharina S. Fischer, MD1Kellen Chen, PhD2Autumn H. Greco, BS3Russell A. Martin, PhD4Dharshan Sivaraj, BS5Artem A. Trotsyuk, BS6Hai-Quan Mao, PhD7Sashank K. Reddy, MD, PhD8Ulrich Kneser, MD9Geoffrey C. Gurtner, MD10Volker J. Schmidt, MD11Justin M. Sacks, MD121 Hagey Laboratory for Pediatric Regenerative Medicine, Div. of Plastic and Reconstructive Surgery, Stanford University, Stanford, CA, USA,1 Hagey Laboratory for Pediatric Regenerative Medicine, Div. of Plastic and Reconstructive Surgery, Stanford University, Stanford, CA, USA,1 Hagey Laboratory for Pediatric Regenerative Medicine, Div. of Plastic and Reconstructive Surgery, Stanford University, Stanford, CA, USA,1 Hagey Laboratory for Pediatric Regenerative Medicine, Div. of Plastic and Reconstructive Surgery, Stanford University, Stanford, CA, USA,2 Department of Materials Science & Engineering, Whiting School of Engineering, and Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA,1 Hagey Laboratory for Pediatric Regenerative Medicine, Div. of Plastic and Reconstructive Surgery, Stanford University, Stanford, CA, USA,1 Hagey Laboratory for Pediatric Regenerative Medicine, Div. of Plastic and Reconstructive Surgery, Stanford University, Stanford, CA, USA,2 Department of Materials Science & Engineering, Whiting School of Engineering, and Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA,1 Hagey Laboratory for Pediatric Regenerative Medicine, Div. of Plastic and Reconstructive Surgery, Stanford University, Stanford, CA, USA,3 Department of Hand, Plastic, and Reconstructive Surgery, BG Trauma Center Ludwigshafen, Ruprecht-Karls-University of Heidelberg, Heidelberg, Heidelberg, Germany,1 Hagey Laboratory for Pediatric Regenerative Medicine, Div. of Plastic and Reconstructive Surgery, Stanford University, Stanford, CA, USA,1 Hagey Laboratory for Pediatric Regenerative Medicine, Div. of Plastic and Reconstructive Surgery, Stanford University, Stanford, CA, USA,4 Division of Plastic and Reconstructive Surgery, Department of Surgery, Washington University in St. Louis School of Medicine, St. Louis, MO, USA.Purpose: Fractionated fat (FF) has been shown to promote dermal regeneration; however, the use of fat grafting for reconstruction of soft tissue defects is limited due to volume loss over time. We have developed a novel approach for engineering of vascularized soft tissue using an injectable nanofiber hydrogel (NHC) enriched with FF. Methods: FF was generated by emulsification of groin fat pads from rats and mixed in a 3:1 ratio with NHC (NHC-FF). NHC-FF or NHC alone were placed into isolation chambers together with arteriovenous (AV) loops, which were subcutaneously implanted into the groin of rats (n=8 per group). After 21 days, animals were euthanized, systemically perfused with ink, and tissue was explanted for histological analysis. Immunofluorescent staining (IF) and confocal laser scanning microscopy were used to quantify CD34+ progenitor cells and macrophage subpopulations. Results: NHC-FF tissue maintained its shape without shrinking and showed a significantly stronger functional neovascularization compared to NHC alone at 21 days after implantation (mean vessel count: 833.5 ± 206.1 vs. 296.5 ± 114.1, p = 0.04). Tissue remodeling and cellular infiltration were greater in NHC-FF (mean cell count: 49,707 ± 18,491 vs. 9,263 ± 3,790, p = 0.005) with a significantly higher amount of progenitor cells and regenerative CD163+ macrophages compared to NHC alone. Conclusion: FF-enriched NHC transforms into highly vascularized soft tissue over 21 days without signs of shrinking and promotes macrophage polarization toward regenerative phenotypes. Enrichment of injectable NHC with FF represents a promising approach for durable reconstruction of soft tissue defects.http://journals.lww.com/prsgo/fulltext/10.1097/01.GOX.0000769980.35543.2a |