Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2).

Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2).

We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic RNA silencing was target-specific and is a new tool for gene regulation and meta...

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Main Authors: Gabriel C Uguru, Madhav Mondhe, Shan Goh, Andrew Hesketh, Mervyn J Bibb, Liam Good, James E M Stach
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3694883?pdf=render
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spelling doaj-eb0d76336fb542e08674459132111c7f2020-11-25T01:31:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0186e6750910.1371/journal.pone.0067509Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2).Gabriel C UguruMadhav MondheShan GohAndrew HeskethMervyn J BibbLiam GoodJames E M StachWe demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic RNA silencing was target-specific and is a new tool for gene regulation and metabolic engineering studies in Streptomyces.http://europepmc.org/articles/PMC3694883?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Gabriel C Uguru
Madhav Mondhe
Shan Goh
Andrew Hesketh
Mervyn J Bibb
Liam Good
James E M Stach
spellingShingle Gabriel C Uguru
Madhav Mondhe
Shan Goh
Andrew Hesketh
Mervyn J Bibb
Liam Good
James E M Stach
Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2).
PLoS ONE
author_facet Gabriel C Uguru
Madhav Mondhe
Shan Goh
Andrew Hesketh
Mervyn J Bibb
Liam Good
James E M Stach
author_sort Gabriel C Uguru
title Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2).
title_short Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2).
title_full Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2).
title_fullStr Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2).
title_full_unstemmed Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2).
title_sort synthetic rna silencing of actinorhodin biosynthesis in streptomyces coelicolor a3(2).
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2013-01-01
description We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic RNA silencing was target-specific and is a new tool for gene regulation and metabolic engineering studies in Streptomyces.
url http://europepmc.org/articles/PMC3694883?pdf=render
work_keys_str_mv AT gabrielcuguru syntheticrnasilencingofactinorhodinbiosynthesisinstreptomycescoelicolora32
AT madhavmondhe syntheticrnasilencingofactinorhodinbiosynthesisinstreptomycescoelicolora32
AT shangoh syntheticrnasilencingofactinorhodinbiosynthesisinstreptomycescoelicolora32
AT andrewhesketh syntheticrnasilencingofactinorhodinbiosynthesisinstreptomycescoelicolora32
AT mervynjbibb syntheticrnasilencingofactinorhodinbiosynthesisinstreptomycescoelicolora32
AT liamgood syntheticrnasilencingofactinorhodinbiosynthesisinstreptomycescoelicolora32
AT jamesemstach syntheticrnasilencingofactinorhodinbiosynthesisinstreptomycescoelicolora32
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