1,2-Bis[(3-methoxyphenyl)methyl]ethane-1,2-Dicarboxylic Acid Reduces UVB-Induced Photodamage In Vitro and In Vivo

• Main Authors: Po-Yuan Wu, Tzu-Yu Lin, Chien-Wei Hou, Qiao-Xin Chang, Kuo-Ching Wen, Chien-Yih Lin, Hsiu-Mei Chiang
• Format: Article
• Language: English
• Published: MDPI AG 2019-10-01
• Series: Antioxidants
• Subjects: 1,2-bis[(3-methoxyphenyl)methyl]ethane-1,2-dicaroxylic acid; photodamage; inflammation; nuclear factor-kappa b; inhibitor κb
• Online Access: https://www.mdpi.com/2076-3921/8/10/452

This study investigated the effects and mechanisms of 1,2-bis[(3-methoxyphenyl)methyl]ethane-1,2-dicarboxylic acid (S4), a sesamin derivative, on anti-inflammation and antiphotoaging in vitro and in vivo. Human skin fibroblasts were treated with S4 and did not show cytotoxicity under concentrations of 5−50 µM. In addition, S4 also reduced ultraviolet (UV)B-induced intracellular reactive oxygen species (ROS) production. Additionally, S4 inhibited UVB-induced phosphorylation of mitogen-activated protein (MAP) kinases, activator protein-1 (AP-1), and matrix metalloproteinases (MMPs) overexpression. Furthermore, S4 also inhibited UVB-induced Smad7 protein expression and elevated total collagen content in human dermal fibroblasts. For anti-inflammatory activity, S4 inhibited UVB-induced nitric oxide synthase (i-NOS) and cyclooxygenase (COX)-2 protein expression and inhibited nuclear factor-kappaB (NF-ĸB) translocation into the nucleus. S4 ameliorated UVB-induced erythema and wrinkle formation in hairless mice. On histological observation, S4 also ameliorated UVB-induced epidermal hyperplasia and collagen degradation. S4 reduced UVB-induced MMP-1, interleukin (IL)-6, and NF-ĸB expression in the mouse skin. The results indicated that S4 had antiphotoaging and anti-inflammatory activities, protecting skin from premature aging.