Expression of MMP-2 and TIMP-1 in Renal Tissue of Patients with Chronic Active Antibody-mediated Renal Graft Rejection

• Main Authors: Yan Qiang, Sui Weiguo, Wang Baoyao, Zou Hequn, Zou Guimian, Luo Hao
• Format: Article
• Language: English
• Published: BMC 2012-10-01
• Series: Diagnostic Pathology
• Subjects: Matrix metalloproteinase-2; Tissue inhibitor of metalloproteinase-1; Chronic active antibody-mediate rejection; Interstitial fibrosis and tubular atrophy
• Online Access: http://www.diagnosticpathology.org/content/7/1/141

<p>Abstract</p> <p>Objective</p> <p>To investigate the expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metallopropteinase-1 (TIMP-1) in the renal allografts of patients with chronic active antibody-mediated rejection (AMR), and to explore their role in the pathogenesis of AMR.</p> <p>Methods</p> <p>Immunohistochemistry assay and computer-assisted image analysis were used to detect the expression of MMP-2 and TIMP-1 in the renal allografts with interstitial fibrosis and tubular atrophy (IF/TA) in 46 transplant recipients and 15 normal renal tissue specimens as the controls. The association of the expression level of either MMP-2 or TIMP-1 with the pathological grade of IF/TA in AMR was analyzed.</p> <p>Results</p> <p>The expression of either MMP-2 or TIMP-1 was significantly increased in the renal allografts of the recipients as compared with the normal renal tissue (<it>P</it> < 0.05). MMP-2 expression tended to decrease, while TIMP-1 and serum creatinine increased along with the increase of pathological grade of IF/TA (<it>P</it> < 0.05). In IF/TA groups, the expression of TIMP-1 was positively correlated to serum creatinine level (r = 0.718, <it>P</it> < 0.05).</p> <p>Conclusions</p> <p>It is suggested by the results that abnormal expressions of MMP-2 and TIMP-1 might play roles in the development of renal fibrosis in chronic AMR.</p> <p>Virtual Slides</p> <p>The virtual slide(s) for this article can be found here: <url>http://www.diagnosticpathology.diagnomx.eu/vs/1128474926172838</url></p>