Scale up Pseudomonas fluorescens-based biofertilizers production

• Main Authors: María Cristina Pérez Peñaranda, Julio Oramas García, Emilio Arcadio Sotolongo Valdés, Jessica Valdivia Pérez, Yoandra Román Tabio, Yoel Beovides García, Jaime Simó González
• Format: Article
• Language: English
• Published: Universidad Central Marta Abreu de Las Villas 2020-12-01
• Series: Biotecnología Vegetal
• Subjects: banana; biofertilizer; scale up; sweet potato
• Online Access: https://revista.ibp.co.cu/index.php/BV/article/view/680

In previous studies, a semi-synthetic culture medium was optimized for the industrial production of a plant growth-promoting strain of Pseudomonas fluorescens. This work was proposed to develop the scale up for producing the biofertilizer. The criteria for scale up were selected taking into account the geometric non-similarity of the fermenters used (42 and 500 liters). The technical effectiveness of the resulting bioproduct was evaluated in liquid formulation on in vitro plants of banana cv. Manzano (Musa AAA) in the acclimatization stage (root immersion 10, 15, 20 and 25 min) and in the field cultivation of Ipomoea batatas applied to the soil at a rate of 20 l ha-1. The results showed that under the conditions tested, bacterial growth was obtained with similar kinetics on two scales. The fermentations concluded 12 hours after the start of the culture. The banana in vitro plants responded better when the immersions in the biofertilizer occurred for 20 min. In the cultivation of sweet potato a positive response was obtained when it was combined with mineral fertilizers and a reduction of 25% of the recommended dose was achieved. The production of a biofertilizer based on Pseudomonas fluorescens can be carried out in a 500 l stirred tank fermenter (effective volume 350 l) for 12 h with stirring speed of 266 rpm and air flow of 105 l min-1 which guarantees a concentration of 1x109 CFU ml-1 and its plant growth promotion effects.