Development of magnet configurations for magnetic immunostaining

Development of magnet configurations for magnetic immunostaining

Magnetic immunostaining using a magnet and antibody-labeled fluorescent ferrite (FF) beads is established as a rapid immunostaining. In this study, we proposed the novel configuration of magnets with the large magnetic field gradient and the strong magnetic force for magnetic immunostaining. To conf...

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Main Authors: Miki Kaneko, Shinichi Chikaki, Sachiko Matsuda, Akihiro Kuwahata, Masayuki Namita, Itsuro Saito, Satoshi Sakamoto, Moriaki Kusakabe, Masaki Sekino
Format: Article
Language:English
Published: AIP Publishing LLC 2018-05-01
Series:AIP Advances
Online Access:http://dx.doi.org/10.1063/1.5007918
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spelling doaj-ea93b3bb637345beb3b235e5752f78182020-11-24T22:47:59ZengAIP Publishing LLCAIP Advances2158-32262018-05-0185056732056732-610.1063/1.5007918293892ADVDevelopment of magnet configurations for magnetic immunostainingMiki Kaneko0Shinichi Chikaki1Sachiko Matsuda2Akihiro Kuwahata3Masayuki Namita4Itsuro Saito5Satoshi Sakamoto6Moriaki Kusakabe7Masaki Sekino8The University of Tokyo, Tokyo, JapanThe University of Tokyo, Tokyo, JapanKeio University, Tokyo, JapanThe University of Tokyo, Tokyo, JapanThe University of Tokyo, Tokyo, JapaniMed Japan Inc., Tokyo, JapanTokyo Institute of Technology, Kanagawa, JapanThe University of Tokyo, Tokyo, JapanThe University of Tokyo, Tokyo, JapanMagnetic immunostaining using a magnet and antibody-labeled fluorescent ferrite (FF) beads is established as a rapid immunostaining. In this study, we proposed the novel configuration of magnets with the large magnetic field gradient and the strong magnetic force for magnetic immunostaining. To confirm the usefulness of the proposed magnet configuration, we performed numerical analysis of the magnetic characteristics of the proposed magnets, and the magnetic immunostaining with FF beads. It was revealed that the proposed magnets generated the strong magnetic force and promoted the immunoreaction rapidly.http://dx.doi.org/10.1063/1.5007918
collection DOAJ
language English
format Article
sources DOAJ
author Miki Kaneko
Shinichi Chikaki
Sachiko Matsuda
Akihiro Kuwahata
Masayuki Namita
Itsuro Saito
Satoshi Sakamoto
Moriaki Kusakabe
Masaki Sekino
spellingShingle Miki Kaneko
Shinichi Chikaki
Sachiko Matsuda
Akihiro Kuwahata
Masayuki Namita
Itsuro Saito
Satoshi Sakamoto
Moriaki Kusakabe
Masaki Sekino
Development of magnet configurations for magnetic immunostaining
AIP Advances
author_facet Miki Kaneko
Shinichi Chikaki
Sachiko Matsuda
Akihiro Kuwahata
Masayuki Namita
Itsuro Saito
Satoshi Sakamoto
Moriaki Kusakabe
Masaki Sekino
author_sort Miki Kaneko
title Development of magnet configurations for magnetic immunostaining
title_short Development of magnet configurations for magnetic immunostaining
title_full Development of magnet configurations for magnetic immunostaining
title_fullStr Development of magnet configurations for magnetic immunostaining
title_full_unstemmed Development of magnet configurations for magnetic immunostaining
title_sort development of magnet configurations for magnetic immunostaining
publisher AIP Publishing LLC
series AIP Advances
issn 2158-3226
publishDate 2018-05-01
description Magnetic immunostaining using a magnet and antibody-labeled fluorescent ferrite (FF) beads is established as a rapid immunostaining. In this study, we proposed the novel configuration of magnets with the large magnetic field gradient and the strong magnetic force for magnetic immunostaining. To confirm the usefulness of the proposed magnet configuration, we performed numerical analysis of the magnetic characteristics of the proposed magnets, and the magnetic immunostaining with FF beads. It was revealed that the proposed magnets generated the strong magnetic force and promoted the immunoreaction rapidly.
url http://dx.doi.org/10.1063/1.5007918
work_keys_str_mv AT mikikaneko developmentofmagnetconfigurationsformagneticimmunostaining
AT shinichichikaki developmentofmagnetconfigurationsformagneticimmunostaining
AT sachikomatsuda developmentofmagnetconfigurationsformagneticimmunostaining
AT akihirokuwahata developmentofmagnetconfigurationsformagneticimmunostaining
AT masayukinamita developmentofmagnetconfigurationsformagneticimmunostaining
AT itsurosaito developmentofmagnetconfigurationsformagneticimmunostaining
AT satoshisakamoto developmentofmagnetconfigurationsformagneticimmunostaining
AT moriakikusakabe developmentofmagnetconfigurationsformagneticimmunostaining
AT masakisekino developmentofmagnetconfigurationsformagneticimmunostaining
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