Development of magnet configurations for magnetic immunostaining

Magnetic immunostaining using a magnet and antibody-labeled fluorescent ferrite (FF) beads is established as a rapid immunostaining. In this study, we proposed the novel configuration of magnets with the large magnetic field gradient and the strong magnetic force for magnetic immunostaining. To conf...

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Bibliographic Details
Main Authors: Miki Kaneko, Shinichi Chikaki, Sachiko Matsuda, Akihiro Kuwahata, Masayuki Namita, Itsuro Saito, Satoshi Sakamoto, Moriaki Kusakabe, Masaki Sekino
Format: Article
Language:English
Published: AIP Publishing LLC 2018-05-01
Series:AIP Advances
Online Access:http://dx.doi.org/10.1063/1.5007918
Description
Summary:Magnetic immunostaining using a magnet and antibody-labeled fluorescent ferrite (FF) beads is established as a rapid immunostaining. In this study, we proposed the novel configuration of magnets with the large magnetic field gradient and the strong magnetic force for magnetic immunostaining. To confirm the usefulness of the proposed magnet configuration, we performed numerical analysis of the magnetic characteristics of the proposed magnets, and the magnetic immunostaining with FF beads. It was revealed that the proposed magnets generated the strong magnetic force and promoted the immunoreaction rapidly.
ISSN:2158-3226