Comparative Mitogenomic Analysis of Two Longhorn Beetles (Coleoptera: Cerambycidae: Lamiinae) with Preliminary Investigation into Phylogenetic Relationships of Tribes of Lamiinae

• Main Authors: Yifang Ren, Huanhuan Lu, Longyan Chen, Simone Sabatelli, Chaojie Wang, Guanglin Xie, Ping Wang, Meike Liu, Wenkai Wang, Paolo Audisio
• Format: Article
• Language: English
• Published: MDPI AG 2021-09-01
• Series: Insects
• Subjects: molecular; mitochondrial genome; genome structure; phylogenetic analysis
• Online Access: https://www.mdpi.com/2075-4450/12/9/820

The subfamily Lamiinae is the most taxonomically diverse subfamily of Cerambycidae, but relationships between tribes of Lamiinae are still unresolved. In order to study the characteristics of the mitogenomes of Lamiinae and the tribal-level phylogenetic relationships, we sequenced the mitogenomes of two species representing two tribes, <i>Agapanthia amurensis</i> (Agapanthiini) and <i>Moechotypa diphysis</i> (Ceroplesini), with a total length of 15,512 bp and 15,493 bp, respectively. The gene arrangements of these two new mitogenomes were consistent with the inferred ancestral insect mitogenomes. Each species contained 37 typical mitochondrial genes and a control region (A + T-rich region), including 13 protein-coding genes (<i>PCGs</i>), 22 transfer RNA genes (tRNAs), and two ribosomal RNA genes (rRNAs). All <i>PCGs</i> initiated with the standard start codon ATN, and terminated with the complete stop codons of TAA and TAG, or incomplete stop codon T. All tRNAs could be folded into a clover-leaf secondary structure except for <i>trnS1</i>, in which the dihydrouridine (DHU) arm was reduced. Moreover, we studied the phylogenetic relationships between some tribes of Lamiinae based in mitochondrial <i>PCGs</i> in nucleotides; our results show that the relationships were as follows: (Onciderini + ((Apomecynini + Acanthocinini) + ((Ceroplesini + Agapanthiini) + ((Mesosini + Pteropliini) + ((Dorcaschematini + (Saperdini 1 + (Phytoeciini + Saperdini 2))) + (Batocerini + Lamiini)))))).