Effects of liraglutide on lipolysis and the AC3/PKA/HSL pathway
Zhengming Li1,*, Pijian Yang2,*, Yuzhen Liang1,*, Ning Xia2,*, Yingrong Li1, Hongye Su1, Hailin Pan1 1Department of Endocrinology and Metabolism, Second Affiliated Hospital of Guangxi Medical University, Nanning, People’s Republic of China; 2Department of Endocrinology and Metabolism, The...
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doaj-ea800619b7954806bd9de5ccd99341802020-11-25T01:46:44ZengDove Medical PressDiabetes, Metabolic Syndrome and Obesity : Targets and Therapy1178-70072019-09-01Volume 121697170348353Effects of liraglutide on lipolysis and the AC3/PKA/HSL pathwayLi ZYang PLiang YXia NLi YSu HPan HZhengming Li1,*, Pijian Yang2,*, Yuzhen Liang1,*, Ning Xia2,*, Yingrong Li1, Hongye Su1, Hailin Pan1 1Department of Endocrinology and Metabolism, Second Affiliated Hospital of Guangxi Medical University, Nanning, People’s Republic of China; 2Department of Endocrinology and Metabolism, The First Affiliated Hospital of Guangxi Medical University, Guangxi Medical University, Nanning, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yuzhen LiangDepartment of Endocrinology and Metabolism, Second Affiliated Hospital of Guangxi Medical University, Nanning 530007, People’s Republic of ChinaEmail liangyuzhen26@163.comNing XiaDepartment of Endocrinology and Metabolism, The First Affiliated Hospital of Guangxi Medical University, Guangxi Medical University, Nanning 530021, People’s Republic of ChinaEmail xianinggxmu@163.comBackground: Liraglutide reduces blood glucose, body weight and blood lipid levels. Hormone-sensitive lipase (HSL) is a key enzyme in lipolysis. Evidence from our and other studies have demonstrated that adenylate cyclase 3 (AC3) is associated with obesity and can be upregulated by liraglutide in obese mice. In the present study, we investigated whether hepatic HSL activity is regulated by liraglutide and characterized the effect of liraglutide in the AC3/protein kinase A (PKA)/HSL signalling pathway.Methods: Obese mice or their lean littermates were treated with liraglutide or saline for 8 weeks. Serum was collected for the measurement of insulin and lipids. We investigated hepatic AC3, HSL and phosphorylated HSL Ser-660 (p-HSL(S660)) protein expression levels andAC3 and HSL mRNA expression levels and cyclic adenosine monophosphate (cAMP), PKA activity in liver tissue.Results: Liraglutide treatment decreased triglycerides (TGs) and free fatty acids (FFAs), increased glycerol, and upregulated hepatic AC3 and p-HSL(s660) levels and cAMP and PKA activities.Conclusion: The results suggest that liraglutide can upregulates AC3/PKA/HSL pathway and may promotes lipolysis.Keywords: liraglutide, obesity, lipolysis, adenylate cyclase 3, hormone-sensitive lipasehttps://www.dovepress.com/effects-of-liraglutide-on-lipolysis-and-the-ac3pkahsl-pathway-peer-reviewed-article-DMSOliraglutideobesitylipolysisadenylate cyclase 3hormone-sensitive lipase |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Li Z Yang P Liang Y Xia N Li Y Su H Pan H |
spellingShingle |
Li Z Yang P Liang Y Xia N Li Y Su H Pan H Effects of liraglutide on lipolysis and the AC3/PKA/HSL pathway Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy liraglutide obesity lipolysis adenylate cyclase 3 hormone-sensitive lipase |
author_facet |
Li Z Yang P Liang Y Xia N Li Y Su H Pan H |
author_sort |
Li Z |
title |
Effects of liraglutide on lipolysis and the AC3/PKA/HSL pathway |
title_short |
Effects of liraglutide on lipolysis and the AC3/PKA/HSL pathway |
title_full |
Effects of liraglutide on lipolysis and the AC3/PKA/HSL pathway |
title_fullStr |
Effects of liraglutide on lipolysis and the AC3/PKA/HSL pathway |
title_full_unstemmed |
Effects of liraglutide on lipolysis and the AC3/PKA/HSL pathway |
title_sort |
effects of liraglutide on lipolysis and the ac3/pka/hsl pathway |
publisher |
Dove Medical Press |
series |
Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy |
issn |
1178-7007 |
publishDate |
2019-09-01 |
description |
Zhengming Li1,*, Pijian Yang2,*, Yuzhen Liang1,*, Ning Xia2,*, Yingrong Li1, Hongye Su1, Hailin Pan1 1Department of Endocrinology and Metabolism, Second Affiliated Hospital of Guangxi Medical University, Nanning, People’s Republic of China; 2Department of Endocrinology and Metabolism, The First Affiliated Hospital of Guangxi Medical University, Guangxi Medical University, Nanning, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yuzhen LiangDepartment of Endocrinology and Metabolism, Second Affiliated Hospital of Guangxi Medical University, Nanning 530007, People’s Republic of ChinaEmail liangyuzhen26@163.comNing XiaDepartment of Endocrinology and Metabolism, The First Affiliated Hospital of Guangxi Medical University, Guangxi Medical University, Nanning 530021, People’s Republic of ChinaEmail xianinggxmu@163.comBackground: Liraglutide reduces blood glucose, body weight and blood lipid levels. Hormone-sensitive lipase (HSL) is a key enzyme in lipolysis. Evidence from our and other studies have demonstrated that adenylate cyclase 3 (AC3) is associated with obesity and can be upregulated by liraglutide in obese mice. In the present study, we investigated whether hepatic HSL activity is regulated by liraglutide and characterized the effect of liraglutide in the AC3/protein kinase A (PKA)/HSL signalling pathway.Methods: Obese mice or their lean littermates were treated with liraglutide or saline for 8 weeks. Serum was collected for the measurement of insulin and lipids. We investigated hepatic AC3, HSL and phosphorylated HSL Ser-660 (p-HSL(S660)) protein expression levels andAC3 and HSL mRNA expression levels and cyclic adenosine monophosphate (cAMP), PKA activity in liver tissue.Results: Liraglutide treatment decreased triglycerides (TGs) and free fatty acids (FFAs), increased glycerol, and upregulated hepatic AC3 and p-HSL(s660) levels and cAMP and PKA activities.Conclusion: The results suggest that liraglutide can upregulates AC3/PKA/HSL pathway and may promotes lipolysis.Keywords: liraglutide, obesity, lipolysis, adenylate cyclase 3, hormone-sensitive lipase |
topic |
liraglutide obesity lipolysis adenylate cyclase 3 hormone-sensitive lipase |
url |
https://www.dovepress.com/effects-of-liraglutide-on-lipolysis-and-the-ac3pkahsl-pathway-peer-reviewed-article-DMSO |
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