Oxymatrine Downregulates HPV16E7 Expression and Inhibits Cell Proliferation in Laryngeal Squamous Cell Carcinoma Hep-2 Cells In Vitro
Objective. To investigate the possible mechanisms of oxymatrine’s role in anti laryngeal squamous cell carcinoma. Methods. We examined the effects of oxymatrine on the proliferation, cell cycle phase distribution, apoptosis, and the protein and mRNA expression levels of HPV16E7 gene in laryngeal car...
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Online Access: | http://dx.doi.org/10.1155/2015/150390 |
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doaj-ea67f2a90df54067b01f2e5106ba74c12020-11-24T22:52:05ZengHindawi LimitedBioMed Research International2314-61332314-61412015-01-01201510.1155/2015/150390150390Oxymatrine Downregulates HPV16E7 Expression and Inhibits Cell Proliferation in Laryngeal Squamous Cell Carcinoma Hep-2 Cells In VitroXin-Jiang Ying0Bin Jin1Xin-Wei Chen2Jin Xie3Hong-Ming Xu4Pin Dong5Department of Otolaryngology-Head and Neck Surgery, Shanghai First People’s Hospital, Shanghai Jiao Tong University, Shanghai 200080, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Shanghai First People’s Hospital, Shanghai Jiao Tong University, Shanghai 200080, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Shanghai First People’s Hospital, Shanghai Jiao Tong University, Shanghai 200080, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Shanghai First People’s Hospital, Shanghai Jiao Tong University, Shanghai 200080, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Shanghai First People’s Hospital, Shanghai Jiao Tong University, Shanghai 200080, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Shanghai First People’s Hospital, Shanghai Jiao Tong University, Shanghai 200080, ChinaObjective. To investigate the possible mechanisms of oxymatrine’s role in anti laryngeal squamous cell carcinoma. Methods. We examined the effects of oxymatrine on the proliferation, cell cycle phase distribution, apoptosis, and the protein and mRNA expression levels of HPV16E7 gene in laryngeal carcinoma Hep-2 cells in vitro. The HPV16E7 siRNA inhibition was also done to confirm the effect of downregulating HPV16E7 on the proliferation in Hep-2 cells. Results. Oxymatrine significantly inhibited the growth and proliferation of Hep-2 cells in a dose-dependence and time-dependence manner. Oxymatrine blocked Hep-2 cells in G0/G1 phase, resulting in an obvious accumulation of G0/G1 phase cells while decreasing S phase cells. Oxymatrine induced apoptosis of Hep-2 cells, whose apoptotic rate amounted to about 42% after treatment with 7 mg/mL oxymatrine for 72 h. Oxymatrine also downregulated the expression of HPV16E7 gene, as determined by the western blotting and reverse transcription-polymerase chain reaction analysis. Knockdown of HPV16E7 effectively inhibited the proliferation of Hep-2 cells. Conclusions. Oxymatrine inhibits the proliferation and induces apoptosis of laryngeal carcinoma Hep-2 cells, which might be mediated by a significant cell cycle arrest in G0/G1 phase and downregulation of HPV16E7 gene. Oxymatrine is considered to be a likely preventive and curative candidate for laryngeal cancer.http://dx.doi.org/10.1155/2015/150390 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xin-Jiang Ying Bin Jin Xin-Wei Chen Jin Xie Hong-Ming Xu Pin Dong |
spellingShingle |
Xin-Jiang Ying Bin Jin Xin-Wei Chen Jin Xie Hong-Ming Xu Pin Dong Oxymatrine Downregulates HPV16E7 Expression and Inhibits Cell Proliferation in Laryngeal Squamous Cell Carcinoma Hep-2 Cells In Vitro BioMed Research International |
author_facet |
Xin-Jiang Ying Bin Jin Xin-Wei Chen Jin Xie Hong-Ming Xu Pin Dong |
author_sort |
Xin-Jiang Ying |
title |
Oxymatrine Downregulates HPV16E7 Expression and Inhibits Cell Proliferation in Laryngeal Squamous Cell Carcinoma Hep-2 Cells In Vitro |
title_short |
Oxymatrine Downregulates HPV16E7 Expression and Inhibits Cell Proliferation in Laryngeal Squamous Cell Carcinoma Hep-2 Cells In Vitro |
title_full |
Oxymatrine Downregulates HPV16E7 Expression and Inhibits Cell Proliferation in Laryngeal Squamous Cell Carcinoma Hep-2 Cells In Vitro |
title_fullStr |
Oxymatrine Downregulates HPV16E7 Expression and Inhibits Cell Proliferation in Laryngeal Squamous Cell Carcinoma Hep-2 Cells In Vitro |
title_full_unstemmed |
Oxymatrine Downregulates HPV16E7 Expression and Inhibits Cell Proliferation in Laryngeal Squamous Cell Carcinoma Hep-2 Cells In Vitro |
title_sort |
oxymatrine downregulates hpv16e7 expression and inhibits cell proliferation in laryngeal squamous cell carcinoma hep-2 cells in vitro |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2015-01-01 |
description |
Objective. To investigate the possible mechanisms of oxymatrine’s role in anti laryngeal squamous cell carcinoma. Methods. We examined the effects of oxymatrine on the proliferation, cell cycle phase distribution, apoptosis, and the protein and mRNA expression levels of HPV16E7 gene in laryngeal carcinoma Hep-2 cells in vitro. The HPV16E7 siRNA inhibition was also done to confirm the effect of downregulating HPV16E7 on the proliferation in Hep-2 cells. Results. Oxymatrine significantly inhibited the growth and proliferation of Hep-2 cells in a dose-dependence and time-dependence manner. Oxymatrine blocked Hep-2 cells in G0/G1 phase, resulting in an obvious accumulation of G0/G1 phase cells while decreasing S phase cells. Oxymatrine induced apoptosis of Hep-2 cells, whose apoptotic rate amounted to about 42% after treatment with 7 mg/mL oxymatrine for 72 h. Oxymatrine also downregulated the expression of HPV16E7 gene, as determined by the western blotting and reverse transcription-polymerase chain reaction analysis. Knockdown of HPV16E7 effectively inhibited the proliferation of Hep-2 cells. Conclusions. Oxymatrine inhibits the proliferation and induces apoptosis of laryngeal carcinoma Hep-2 cells, which might be mediated by a significant cell cycle arrest in G0/G1 phase and downregulation of HPV16E7 gene. Oxymatrine is considered to be a likely preventive and curative candidate for laryngeal cancer. |
url |
http://dx.doi.org/10.1155/2015/150390 |
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