Cell culture conditions affect the ability of high content imaging assay to detect drug-induced changes in cellular parameters in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)

Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are widely used for drug safety and efficacy testing with various techniques, including high content imaging (HCI). Upon drug treatment, a significant number of hiPSC-CMs grown in regular 96-well plates coated with fibronectin de...

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Main Authors: Bharathi Balasubramanian, Vaclav Belak, Isha Verma, Yeva Prysiazhniuk, Frederick Sannajust, Elena S. Trepakova
Format: Article
Language:English
Published: Elsevier 2019-01-01
Series:Toxicology Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2214750018306279
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spelling doaj-ea2baef71dd1402eb01498611f08e68a2020-11-25T01:21:17ZengElsevierToxicology Reports2214-75002019-01-016305320Cell culture conditions affect the ability of high content imaging assay to detect drug-induced changes in cellular parameters in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)Bharathi Balasubramanian0Vaclav Belak1Isha Verma2Yeva Prysiazhniuk3Frederick Sannajust4Elena S. Trepakova5Department of Safety and Exploratory Pharmacology, Safety Assessment and Laboratory Animal Resources, MRL, Merck & Co., Inc, West Point, PA, USADeparment of Data Science, MSD Global IT Innovation Center, Prague, Czech RepublicDepartment of Data Development, Informatics & Analytics, Palo Alto, CA, USADeparment of Data Science, MSD Global IT Innovation Center, Prague, Czech RepublicDepartment of Safety and Exploratory Pharmacology, Safety Assessment and Laboratory Animal Resources, MRL, Merck & Co., Inc, West Point, PA, USADepartment of Safety and Exploratory Pharmacology, Safety Assessment and Laboratory Animal Resources, MRL, Merck & Co., Inc, West Point, PA, USA; Corresponding author at: 770 Sumneytown Pike, PO Box 04, WP81-220, West Point, PA, 19486, USA.Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are widely used for drug safety and efficacy testing with various techniques, including high content imaging (HCI). Upon drug treatment, a significant number of hiPSC-CMs grown in regular 96-well plates coated with fibronectin detached from the bottom of the plate, complicating data acquisition. Several cell culture configurations were tested to improve cell adherence, and the effects of these configurations on total cell number, separation of feature values between the negative (DMSO 0.1%) and positive (antimycin, staurosporine) controls, scale of feature value differences, and data variability were statistically calculated. hiPSC-CMs were plated on fibronectin- (in “blanket” configuration) or MaxGel- (in “sandwich” configuration) coated plates and covered with a layer of either HydroMatrix or MaxGel 2, 7, or 11d after plating. After a total of 14d in culture, cells were treated with compounds, labeled with four fluorescent dyes (Hoechst, TMRM, NucView, and RedDot), and imaged with GE INCell2000. Based on the statistical parameters calculated, the MaxGel 25% 7d “sandwich” was superior to all other tested conditions when the cells were treated with 0.3 μM antimycin for 2 h and test compounds 10 μM crizotinib and 30 μM amiodarone for 48 h. For staurosporine treatment, the best culturing condition varied between MaxGel “sandwich” systems, depending on which parameters were under consideration. Thus, cell culturing conditions can significantly affect the ability of high content imaging to detect changes in cellular features during compound treatment and should be thoroughly evaluated before committing to compound testing. Keywords: Human induced pluripotent stem cell-derived cardiomyocytes, hiPSC-CMs, High content imaging, Cell culturehttp://www.sciencedirect.com/science/article/pii/S2214750018306279
collection DOAJ
language English
format Article
sources DOAJ
author Bharathi Balasubramanian
Vaclav Belak
Isha Verma
Yeva Prysiazhniuk
Frederick Sannajust
Elena S. Trepakova
spellingShingle Bharathi Balasubramanian
Vaclav Belak
Isha Verma
Yeva Prysiazhniuk
Frederick Sannajust
Elena S. Trepakova
Cell culture conditions affect the ability of high content imaging assay to detect drug-induced changes in cellular parameters in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)
Toxicology Reports
author_facet Bharathi Balasubramanian
Vaclav Belak
Isha Verma
Yeva Prysiazhniuk
Frederick Sannajust
Elena S. Trepakova
author_sort Bharathi Balasubramanian
title Cell culture conditions affect the ability of high content imaging assay to detect drug-induced changes in cellular parameters in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)
title_short Cell culture conditions affect the ability of high content imaging assay to detect drug-induced changes in cellular parameters in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)
title_full Cell culture conditions affect the ability of high content imaging assay to detect drug-induced changes in cellular parameters in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)
title_fullStr Cell culture conditions affect the ability of high content imaging assay to detect drug-induced changes in cellular parameters in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)
title_full_unstemmed Cell culture conditions affect the ability of high content imaging assay to detect drug-induced changes in cellular parameters in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)
title_sort cell culture conditions affect the ability of high content imaging assay to detect drug-induced changes in cellular parameters in human induced pluripotent stem cell-derived cardiomyocytes (hipsc-cms)
publisher Elsevier
series Toxicology Reports
issn 2214-7500
publishDate 2019-01-01
description Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are widely used for drug safety and efficacy testing with various techniques, including high content imaging (HCI). Upon drug treatment, a significant number of hiPSC-CMs grown in regular 96-well plates coated with fibronectin detached from the bottom of the plate, complicating data acquisition. Several cell culture configurations were tested to improve cell adherence, and the effects of these configurations on total cell number, separation of feature values between the negative (DMSO 0.1%) and positive (antimycin, staurosporine) controls, scale of feature value differences, and data variability were statistically calculated. hiPSC-CMs were plated on fibronectin- (in “blanket” configuration) or MaxGel- (in “sandwich” configuration) coated plates and covered with a layer of either HydroMatrix or MaxGel 2, 7, or 11d after plating. After a total of 14d in culture, cells were treated with compounds, labeled with four fluorescent dyes (Hoechst, TMRM, NucView, and RedDot), and imaged with GE INCell2000. Based on the statistical parameters calculated, the MaxGel 25% 7d “sandwich” was superior to all other tested conditions when the cells were treated with 0.3 μM antimycin for 2 h and test compounds 10 μM crizotinib and 30 μM amiodarone for 48 h. For staurosporine treatment, the best culturing condition varied between MaxGel “sandwich” systems, depending on which parameters were under consideration. Thus, cell culturing conditions can significantly affect the ability of high content imaging to detect changes in cellular features during compound treatment and should be thoroughly evaluated before committing to compound testing. Keywords: Human induced pluripotent stem cell-derived cardiomyocytes, hiPSC-CMs, High content imaging, Cell culture
url http://www.sciencedirect.com/science/article/pii/S2214750018306279
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