An experimental study on cervix cancer with combination of HSV-TK/GCV suicide gene therapy system and ⁶⁰Co radiotherapy

• Main Authors: Tang Qiusha, Chen Daozhen
• Format: Article
• Language: English
• Published: BMC 2010-11-01
• Series: BMC Cancer
• Online Access: http://www.biomedcentral.com/1471-2407/10/609

<p>Abstract</p> <p>Background</p> <p>To evaluate the killing effect of HSV-TK/GCV suicide gene therapy system combined with ⁶⁰Co radiotherapy on human cervical cancer Hela cell line <it>in vitro </it>and <it>in vivo</it>, and to explore the radiosensitization by HSV-TK/GCV system.</p> <p>Methods</p> <p>HSV-TK/GCV suicide gene therapy system and ⁶⁰Co radiotherapy were used separately or in combination on human cervical cancer Hela cell line <it>in vitro </it>and <it>in vivo </it>to compare their effects. Colony formation test and the rate of radiosensitization effect (E/O) were employed to observed the radiosensitization by HSV-TK/GCV system.</p> <p>Results</p> <p>HSV-TK/GCV suicide gene therapy system had strong therapeutic effects on Hela cells <it>in vitro </it>and <it>in vivo </it>(the inhibition rates were 45.8% and 39.5%, respectively), moreover, the combined administration of gene therapy and radiotherapy had stronger therapeutic effects <it>in vitro </it>and <it>in vivo </it>(the inhibition rate was 87.5% <b><it>in vitro</it></b>, and the inhibition rate was 87.9% <it>in vivo</it>) (P < 0.01). The inhibition rate by radiotherapy alone was 42.4% <b><it>in vitro </it></b>and 35.8% <it>in vivo</it>. The sensitivity of combined therapy to radiotherapy increased more than that of therapy alone, the ability of colony formation decreased (P < 0.01). The rate of radiosensitivity effect (E/O) was 3.17(> 1.4), indicating HSV-TK/GCV system could exert a sensitizing effect on ⁶⁰Co radiotherapy of the transplanted human cervical cancer cell in nude mice.</p> <p>Conclusion</p> <p>HSV-TK/GCV system had radiosensitization. Gene therapy combined with radiotherapy may be a good supplementary method for cervix cancer synthetic treatment.</p>