Melatonin-Measurement Methods and the Factors Modifying the Results. A Systematic Review of the Literature

• Main Authors: Beata Rzepka-Migut, Justyna Paprocka
• Format: Article
• Language: English
• Published: MDPI AG 2020-03-01
• Series: International Journal of Environmental Research and Public Health
• Subjects: melatonin; measurement methods; body fluids; drugs; dlmo; dim light melatonin onset
• Online Access: https://www.mdpi.com/1660-4601/17/6/1916

Melatonin plays an important role in regulating the sleep−wake cycle and adaptation to environmental changes. Concentration measurements in bioliquids such as serum/plasma, saliva and urine are widely used to assess peripheral rhythm. The aim of the study was to compare methods and conditions of determinations carried out with the identification of factors potentially affecting the measurements obtained. We have identified a group of modifiable and unmodifiable factors that facilitate data interpretation. Knowledge of modifiers allows you to carefully plan the test protocol and then compare the results. There is no one universal sampling standard, because the choice of method and biofluid depends on the purpose of the study and the research group.