In Vivo Differentiation of Human Amniotic Epithelial Cells into Cardiomyocyte-Like Cells and Cell Transplantation Effect on Myocardial Infarction in Rats: Comparison with Cord Blood and Adipose Tissue-Derived Mesenchymal Stem Cells

Human amniotic epithelial cells (h-AECs), which have various merits as a cell source for cell therapy, are known to differentiate into cardiomyocytes in vitro. However, the ability of h-AECs to differentiate into cardiomyocytes in vivo and their cell transplantation effects on myocardial infarction...

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Main Authors: Cheng-Hu Fang, Jiyong Jin, Jun-Ho Joe, Yi-Sun Song, Byung-Im So, Sang Moo Lim, Gi Jeong Cheon, Sang-Keun Woo, Jeong-Chan Ra, Young-Yiul Lee, Kyung-Soo Kim M.D., Ph.D.
Format: Article
Language:English
Published: SAGE Publishing 2012-08-01
Series:Cell Transplantation
Online Access:https://doi.org/10.3727/096368912X653039
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spelling doaj-e9e0aa7fe5e44c5e89fd624c1e1b0f272020-11-25T03:16:20ZengSAGE PublishingCell Transplantation0963-68971555-38922012-08-012110.3727/096368912X653039In Vivo Differentiation of Human Amniotic Epithelial Cells into Cardiomyocyte-Like Cells and Cell Transplantation Effect on Myocardial Infarction in Rats: Comparison with Cord Blood and Adipose Tissue-Derived Mesenchymal Stem CellsCheng-Hu Fang0Jiyong Jin1Jun-Ho Joe2Yi-Sun Song3Byung-Im So4Sang Moo Lim5Gi Jeong Cheon6Sang-Keun Woo7Jeong-Chan Ra8Young-Yiul Lee9Kyung-Soo Kim M.D., Ph.D.10 Division of Cardiology, Yanbian University, Yanji, China Division of Cardiology, Yanbian University, Yanji, China Department of Biomedical Sciences, Hanyang University, Seoul, South Korea Department of Biomedical Sciences, Hanyang University, Seoul, South Korea Department of Biomedical Sciences, Hanyang University, Seoul, South Korea Department of Nuclear Medicine, Korea Cancer Center Hospital, Seoul, South Korea Department of Nuclear Medicine, Korea Cancer Center Hospital, Seoul, South Korea Laboratory of Nuclear Medicine Research, Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, South Korea Stem Cell Research Center, RNLBIO Co., Ltd., Seoul, South Korea Division of Hematology/Oncology, Hanyang University College of Medicine, Seoul, South Korea Division of Cardiology, Hanyang University College of Medicine, Seoul, South KoreaHuman amniotic epithelial cells (h-AECs), which have various merits as a cell source for cell therapy, are known to differentiate into cardiomyocytes in vitro. However, the ability of h-AECs to differentiate into cardiomyocytes in vivo and their cell transplantation effects on myocardial infarction are still unknown. In this study, we assessed whether h-AECs could differentiate into cardiomyocytes in vivo and whether h-AECs transplantation can decrease infarct size and improve cardiac function, in comparison to transplantation of cord blood-derived mesenchymal stem cells (MSCs) or adipose tissue-derived MSCs. For our study, we injected h-AECs, cord blood-derived MSCs, adipose tissue-derived MSCs, and saline into areas of myocardial infarction in athymic nude rats. After 4 weeks, 3% of the surviving h-AECs expressed myosin heavy chain, a marker specific to the myocardium. Compared with the saline group, all cell-implanted groups showed a higher ejection fraction, lower infarct area by positron emission tomography and histology, and more abundant myocardial gene and protein expression in the infarct area. We showed that h-AECs can differentiate into cardiomyocyte-like cells, decrease infarct size, and improve cardiac function in vivo. The beneficial effects of h-AECs were comparable to those of cord blood and adipose tissue-derived MSCs. These results support the need for further studies of h-AECs as a cell source for myocardial regeneration due to their plentiful availability, low immunity, and lack of ethical issues related to their use.https://doi.org/10.3727/096368912X653039
collection DOAJ
language English
format Article
sources DOAJ
author Cheng-Hu Fang
Jiyong Jin
Jun-Ho Joe
Yi-Sun Song
Byung-Im So
Sang Moo Lim
Gi Jeong Cheon
Sang-Keun Woo
Jeong-Chan Ra
Young-Yiul Lee
Kyung-Soo Kim M.D., Ph.D.
spellingShingle Cheng-Hu Fang
Jiyong Jin
Jun-Ho Joe
Yi-Sun Song
Byung-Im So
Sang Moo Lim
Gi Jeong Cheon
Sang-Keun Woo
Jeong-Chan Ra
Young-Yiul Lee
Kyung-Soo Kim M.D., Ph.D.
In Vivo Differentiation of Human Amniotic Epithelial Cells into Cardiomyocyte-Like Cells and Cell Transplantation Effect on Myocardial Infarction in Rats: Comparison with Cord Blood and Adipose Tissue-Derived Mesenchymal Stem Cells
Cell Transplantation
author_facet Cheng-Hu Fang
Jiyong Jin
Jun-Ho Joe
Yi-Sun Song
Byung-Im So
Sang Moo Lim
Gi Jeong Cheon
Sang-Keun Woo
Jeong-Chan Ra
Young-Yiul Lee
Kyung-Soo Kim M.D., Ph.D.
author_sort Cheng-Hu Fang
title In Vivo Differentiation of Human Amniotic Epithelial Cells into Cardiomyocyte-Like Cells and Cell Transplantation Effect on Myocardial Infarction in Rats: Comparison with Cord Blood and Adipose Tissue-Derived Mesenchymal Stem Cells
title_short In Vivo Differentiation of Human Amniotic Epithelial Cells into Cardiomyocyte-Like Cells and Cell Transplantation Effect on Myocardial Infarction in Rats: Comparison with Cord Blood and Adipose Tissue-Derived Mesenchymal Stem Cells
title_full In Vivo Differentiation of Human Amniotic Epithelial Cells into Cardiomyocyte-Like Cells and Cell Transplantation Effect on Myocardial Infarction in Rats: Comparison with Cord Blood and Adipose Tissue-Derived Mesenchymal Stem Cells
title_fullStr In Vivo Differentiation of Human Amniotic Epithelial Cells into Cardiomyocyte-Like Cells and Cell Transplantation Effect on Myocardial Infarction in Rats: Comparison with Cord Blood and Adipose Tissue-Derived Mesenchymal Stem Cells
title_full_unstemmed In Vivo Differentiation of Human Amniotic Epithelial Cells into Cardiomyocyte-Like Cells and Cell Transplantation Effect on Myocardial Infarction in Rats: Comparison with Cord Blood and Adipose Tissue-Derived Mesenchymal Stem Cells
title_sort in vivo differentiation of human amniotic epithelial cells into cardiomyocyte-like cells and cell transplantation effect on myocardial infarction in rats: comparison with cord blood and adipose tissue-derived mesenchymal stem cells
publisher SAGE Publishing
series Cell Transplantation
issn 0963-6897
1555-3892
publishDate 2012-08-01
description Human amniotic epithelial cells (h-AECs), which have various merits as a cell source for cell therapy, are known to differentiate into cardiomyocytes in vitro. However, the ability of h-AECs to differentiate into cardiomyocytes in vivo and their cell transplantation effects on myocardial infarction are still unknown. In this study, we assessed whether h-AECs could differentiate into cardiomyocytes in vivo and whether h-AECs transplantation can decrease infarct size and improve cardiac function, in comparison to transplantation of cord blood-derived mesenchymal stem cells (MSCs) or adipose tissue-derived MSCs. For our study, we injected h-AECs, cord blood-derived MSCs, adipose tissue-derived MSCs, and saline into areas of myocardial infarction in athymic nude rats. After 4 weeks, 3% of the surviving h-AECs expressed myosin heavy chain, a marker specific to the myocardium. Compared with the saline group, all cell-implanted groups showed a higher ejection fraction, lower infarct area by positron emission tomography and histology, and more abundant myocardial gene and protein expression in the infarct area. We showed that h-AECs can differentiate into cardiomyocyte-like cells, decrease infarct size, and improve cardiac function in vivo. The beneficial effects of h-AECs were comparable to those of cord blood and adipose tissue-derived MSCs. These results support the need for further studies of h-AECs as a cell source for myocardial regeneration due to their plentiful availability, low immunity, and lack of ethical issues related to their use.
url https://doi.org/10.3727/096368912X653039
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