In Vivo Differentiation of Human Amniotic Epithelial Cells into Cardiomyocyte-Like Cells and Cell Transplantation Effect on Myocardial Infarction in Rats: Comparison with Cord Blood and Adipose Tissue-Derived Mesenchymal Stem Cells
Human amniotic epithelial cells (h-AECs), which have various merits as a cell source for cell therapy, are known to differentiate into cardiomyocytes in vitro. However, the ability of h-AECs to differentiate into cardiomyocytes in vivo and their cell transplantation effects on myocardial infarction...
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doaj-e9e0aa7fe5e44c5e89fd624c1e1b0f272020-11-25T03:16:20ZengSAGE PublishingCell Transplantation0963-68971555-38922012-08-012110.3727/096368912X653039In Vivo Differentiation of Human Amniotic Epithelial Cells into Cardiomyocyte-Like Cells and Cell Transplantation Effect on Myocardial Infarction in Rats: Comparison with Cord Blood and Adipose Tissue-Derived Mesenchymal Stem CellsCheng-Hu Fang0Jiyong Jin1Jun-Ho Joe2Yi-Sun Song3Byung-Im So4Sang Moo Lim5Gi Jeong Cheon6Sang-Keun Woo7Jeong-Chan Ra8Young-Yiul Lee9Kyung-Soo Kim M.D., Ph.D.10 Division of Cardiology, Yanbian University, Yanji, China Division of Cardiology, Yanbian University, Yanji, China Department of Biomedical Sciences, Hanyang University, Seoul, South Korea Department of Biomedical Sciences, Hanyang University, Seoul, South Korea Department of Biomedical Sciences, Hanyang University, Seoul, South Korea Department of Nuclear Medicine, Korea Cancer Center Hospital, Seoul, South Korea Department of Nuclear Medicine, Korea Cancer Center Hospital, Seoul, South Korea Laboratory of Nuclear Medicine Research, Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, South Korea Stem Cell Research Center, RNLBIO Co., Ltd., Seoul, South Korea Division of Hematology/Oncology, Hanyang University College of Medicine, Seoul, South Korea Division of Cardiology, Hanyang University College of Medicine, Seoul, South KoreaHuman amniotic epithelial cells (h-AECs), which have various merits as a cell source for cell therapy, are known to differentiate into cardiomyocytes in vitro. However, the ability of h-AECs to differentiate into cardiomyocytes in vivo and their cell transplantation effects on myocardial infarction are still unknown. In this study, we assessed whether h-AECs could differentiate into cardiomyocytes in vivo and whether h-AECs transplantation can decrease infarct size and improve cardiac function, in comparison to transplantation of cord blood-derived mesenchymal stem cells (MSCs) or adipose tissue-derived MSCs. For our study, we injected h-AECs, cord blood-derived MSCs, adipose tissue-derived MSCs, and saline into areas of myocardial infarction in athymic nude rats. After 4 weeks, 3% of the surviving h-AECs expressed myosin heavy chain, a marker specific to the myocardium. Compared with the saline group, all cell-implanted groups showed a higher ejection fraction, lower infarct area by positron emission tomography and histology, and more abundant myocardial gene and protein expression in the infarct area. We showed that h-AECs can differentiate into cardiomyocyte-like cells, decrease infarct size, and improve cardiac function in vivo. The beneficial effects of h-AECs were comparable to those of cord blood and adipose tissue-derived MSCs. These results support the need for further studies of h-AECs as a cell source for myocardial regeneration due to their plentiful availability, low immunity, and lack of ethical issues related to their use.https://doi.org/10.3727/096368912X653039 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cheng-Hu Fang Jiyong Jin Jun-Ho Joe Yi-Sun Song Byung-Im So Sang Moo Lim Gi Jeong Cheon Sang-Keun Woo Jeong-Chan Ra Young-Yiul Lee Kyung-Soo Kim M.D., Ph.D. |
spellingShingle |
Cheng-Hu Fang Jiyong Jin Jun-Ho Joe Yi-Sun Song Byung-Im So Sang Moo Lim Gi Jeong Cheon Sang-Keun Woo Jeong-Chan Ra Young-Yiul Lee Kyung-Soo Kim M.D., Ph.D. In Vivo Differentiation of Human Amniotic Epithelial Cells into Cardiomyocyte-Like Cells and Cell Transplantation Effect on Myocardial Infarction in Rats: Comparison with Cord Blood and Adipose Tissue-Derived Mesenchymal Stem Cells Cell Transplantation |
author_facet |
Cheng-Hu Fang Jiyong Jin Jun-Ho Joe Yi-Sun Song Byung-Im So Sang Moo Lim Gi Jeong Cheon Sang-Keun Woo Jeong-Chan Ra Young-Yiul Lee Kyung-Soo Kim M.D., Ph.D. |
author_sort |
Cheng-Hu Fang |
title |
In Vivo Differentiation of Human Amniotic Epithelial Cells into Cardiomyocyte-Like Cells and Cell Transplantation Effect on Myocardial Infarction in Rats: Comparison with Cord Blood and Adipose Tissue-Derived Mesenchymal Stem Cells |
title_short |
In Vivo Differentiation of Human Amniotic Epithelial Cells into Cardiomyocyte-Like Cells and Cell Transplantation Effect on Myocardial Infarction in Rats: Comparison with Cord Blood and Adipose Tissue-Derived Mesenchymal Stem Cells |
title_full |
In Vivo Differentiation of Human Amniotic Epithelial Cells into Cardiomyocyte-Like Cells and Cell Transplantation Effect on Myocardial Infarction in Rats: Comparison with Cord Blood and Adipose Tissue-Derived Mesenchymal Stem Cells |
title_fullStr |
In Vivo Differentiation of Human Amniotic Epithelial Cells into Cardiomyocyte-Like Cells and Cell Transplantation Effect on Myocardial Infarction in Rats: Comparison with Cord Blood and Adipose Tissue-Derived Mesenchymal Stem Cells |
title_full_unstemmed |
In Vivo Differentiation of Human Amniotic Epithelial Cells into Cardiomyocyte-Like Cells and Cell Transplantation Effect on Myocardial Infarction in Rats: Comparison with Cord Blood and Adipose Tissue-Derived Mesenchymal Stem Cells |
title_sort |
in vivo differentiation of human amniotic epithelial cells into cardiomyocyte-like cells and cell transplantation effect on myocardial infarction in rats: comparison with cord blood and adipose tissue-derived mesenchymal stem cells |
publisher |
SAGE Publishing |
series |
Cell Transplantation |
issn |
0963-6897 1555-3892 |
publishDate |
2012-08-01 |
description |
Human amniotic epithelial cells (h-AECs), which have various merits as a cell source for cell therapy, are known to differentiate into cardiomyocytes in vitro. However, the ability of h-AECs to differentiate into cardiomyocytes in vivo and their cell transplantation effects on myocardial infarction are still unknown. In this study, we assessed whether h-AECs could differentiate into cardiomyocytes in vivo and whether h-AECs transplantation can decrease infarct size and improve cardiac function, in comparison to transplantation of cord blood-derived mesenchymal stem cells (MSCs) or adipose tissue-derived MSCs. For our study, we injected h-AECs, cord blood-derived MSCs, adipose tissue-derived MSCs, and saline into areas of myocardial infarction in athymic nude rats. After 4 weeks, 3% of the surviving h-AECs expressed myosin heavy chain, a marker specific to the myocardium. Compared with the saline group, all cell-implanted groups showed a higher ejection fraction, lower infarct area by positron emission tomography and histology, and more abundant myocardial gene and protein expression in the infarct area. We showed that h-AECs can differentiate into cardiomyocyte-like cells, decrease infarct size, and improve cardiac function in vivo. The beneficial effects of h-AECs were comparable to those of cord blood and adipose tissue-derived MSCs. These results support the need for further studies of h-AECs as a cell source for myocardial regeneration due to their plentiful availability, low immunity, and lack of ethical issues related to their use. |
url |
https://doi.org/10.3727/096368912X653039 |
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