Photobleaching Kinetics and Time-Integrated Emission of Fluorescent Probes in Cellular Membranes

Photobleaching Kinetics and Time-Integrated Emission of Fluorescent Probes in Cellular Membranes

Since the pioneering work of Hirschfeld, it is known that time-integrated emission (TiEm) of a fluorophore is independent of fluorescence quantum yield and illumination intensity. Practical implementation of this important result for determining exact probe distribution in living cells is often hamp...

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Bibliographic Details
Main Authors: Daniel Wüstner, Tanja Christensen, Lukasz M. Solanko, Daniel Sage
Format: Article
Language:English
Published: MDPI AG 2014-07-01
Series:Molecules
Subjects:
photophysics
kinetics
autofluorescence correction
cholesterol
BODIPY
transport
Online Access:http://www.mdpi.com/1420-3049/19/8/11096

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