Hypertonic saline solution reduces the inflammatory response in endotoxemic rats

• Main Authors: Mariana Cardillo Theobaldo, Hermes Vieira Barbeiro, Denise Frediani Barbeiro, Ricardo Petroni, Francisco Garcia Soriano
• Format: Article
• Language: English
• Published: Faculdade de Medicina / USP 2012-12-01
• Series: Clinics
• Subjects: LPS; Hypertonic Solution; Interleukin; Oxidative Stress; Intestine
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322012001200018
• ISSN: 1807-5932; 1980-5322

OBJECTIVE: Volume replacement in septic patients improves hemodynamic stability. This effect can reduce the inflammatory response. The objective of this study was to evaluate the effect of 7.5% hypertonic saline solution versus 0.9% normal saline solution for volume replacement during an inflammatory response in endotoxemic rats. METHODS: We measured cytokines (serum and gut), nitrite, and lipid peroxidation (TBARS) as indicators of oxidative stress in the gut. Rats were divided into four groups: control group (C) that did not receive lipopolysaccharide; lipopolysaccharide injection without treatment (LPS); lipopolysaccharide injection with saline treatment (LPS +S); and lipopolysaccharide injection with hypertonic saline treatment (LPS +H). Serum and intestine were collected. Measurements were taken at 1.5, 8, and 24 h after lipopolysaccharide administration. RESULTS: Of the four groups, the LPS +H group had the highest survival rate. Hypertonic saline solution treatment led to lower levels of IL-6, IL-10, nitric oxide, and thiobarbituric acid reactive substances compared to 0.9% normal saline. In addition, hypertonic saline treatment resulted in a lower mortality compared to 0.9% normal saline treatment in endotoxemic rats. Volume replacement reduced levels of inflammatory mediators in the plasma and gut. CONCLUSION: Hypertonic saline treatment reduced mortality and lowered levels of inflammatory mediators in endotoxemic rats. Hypertonic saline also has the advantage of requiring less volume replacement.