EVALUATION OF METHODS FOR PRESERVING Corynespora cassiicola (Berk. & M.A. Curtis) C.T. Wei ISOLATES AVALIAÇÃO DE MÉTODOS PARA PRESERVAÇÃO DE ISOLADOS DE Corynespora cassiicola (Berk. & M.A. Curtis) C.T. Wei

Corynespora cassiicola (Berk. & M.A. Curtis) C.T. Wei is the causal agent for target spot on several economically important crops. So, studies on the C. cassiicola isolates preservation are necessary to maintain mycelium growth and sporulation, during experiments. The objective of this s...

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Bibliographic Details
Main Authors: Marise Cagnin Martins, Adriana Teramoto, Marcos Gomes Cunha
Format: Article
Language:English
Published: Universidade Federal de Goiás 2011-04-01
Series:Pesquisa Agropecuária Tropical
Subjects:
Online Access:http://www.revistas.ufg.br/index.php/pat/article/view/12571
Description
Summary:Corynespora cassiicola (Berk. &amp;amp; M.A. Curtis) C.T. Wei is the causal agent for target spot on several economically important crops. So, studies on the C. cassiicola isolates preservation are necessary to maintain mycelium growth and sporulation, during experiments. The objective of this study was to evaluate the efficiency of three isolates preserving methods: Castellani, periodical transference, and mineral oil, during six months of storage. The evaluations were carried out by analyzing mycelial growth on potato-dextrose-agar medium. It was observed that mycelial growth was higher in the periodical transference method, sporulation was a little higher in the Castellani method, and all methods preserved isolates pathogenicity. Thus, for that phythopatogen, the Castellani method is the most recommended one.<br>&lt;p style="text-align: justify !important;"&gt;&lt;span style="color: #000000;"&gt;&lt;span style="font-family: Verdana, sans-serif;"&gt;&lt;span style="font-size: x-small;"&gt;&lt;span&gt;O fungo Corynespora cassiicola (Berk. &amp;amp; M.A. Curtis) C.T. Wei é o agente causal da mancha alvo, em diversas culturas de importância econômica. Assim, estudos de preservação de isolados de C. cassiicola, que possam manter viáveis isolados do patógeno, durante experimentos, são necessários. O objetivo deste trabalho foi testar a eficiência de três métodos de preservação de isolados: Castellani, repicagens periódicas e óleo mineral, durante seis meses de armazenamento. As avaliações foram efetuadas por meio de análise do desenvolvimento do fungo, em meio de cultivo contendo batata-dextrose-ágar. Constatou-se que o crescimento micelial foi maior para o método de repicagens periódicas; a esporulação, no método Castellani, foi um pouco superior à dos demais métodos; e todos os métodos propiciaram a preservação da patogenicidade dos isolados estudados. Desta forma, recomenda-se, para este fitopatógeno, o método de preservação Castellani.&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;/p&gt; &lt;p class="western"&gt;&lt;br /&gt;&lt;br /&gt;&lt;/p&gt;&lt;br /&gt;
ISSN:1983-4063