Local dimensionality determines imaging speed in localization microscopy

Localisation microscopy enables nanometre-scale imaging of biological samples, but the method is too slow to use on dynamic systems. Here, the authors develop a mathematical model that optimises the number of frames required and estimates the maximum speed for super-resolution imaging.

Bibliographic Details
Main Authors: Patrick Fox-Roberts, Richard Marsh, Karin Pfisterer, Asier Jayo, Maddy Parsons, Susan Cox
Format: Article
Language:English
Published: Nature Publishing Group 2017-01-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/ncomms13558
Description
Summary:Localisation microscopy enables nanometre-scale imaging of biological samples, but the method is too slow to use on dynamic systems. Here, the authors develop a mathematical model that optimises the number of frames required and estimates the maximum speed for super-resolution imaging.
ISSN:2041-1723