Optimized Enzymatic Synthesis of Feruloyl Derivatives Catalyzed by Three Novel Feruloyl Esterases from Talaromyces wortmannii in Detergentless Microemulsions

Three novel feruloyl esterases (Fae125, Fae7262 and Fae68) from Talaromyces wortmannii overexpressed in the C1 platform were evaluated for the transesterification of vinyl ferulate with two acceptors of different size and lipophilicity (prenol and L-arabinose) in detergentless microemulsions. The ef...

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Main Authors:	Io Antonopoulou, Laura Iancu, Peter Jütten, Alexander Piechot, Ulrika Rova, Paul Christakopoulos
Format:	Article
Language:	English
Published:	Elsevier 2018-01-01
Series:	Computational and Structural Biotechnology Journal
Online Access:	http://www.sciencedirect.com/science/article/pii/S2001037018300941

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spelling	doaj-e970980321684260abfb8b76968b5bcb2020-11-24T23:49:10ZengElsevierComputational and Structural Biotechnology Journal2001-03702018-01-0116361369Optimized Enzymatic Synthesis of Feruloyl Derivatives Catalyzed by Three Novel Feruloyl Esterases from Talaromyces wortmannii in Detergentless MicroemulsionsIo Antonopoulou0Laura Iancu1Peter Jütten2Alexander Piechot3Ulrika Rova4Paul Christakopoulos5Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, Luleå SE-97187, SwedenDupont Industrial Biosciences, Nieuwe Kanaal 7-S, Wageningen 6709, the NetherlandsTaros Chemicals GmbH & Co KG, Emil-Figge-Str. 76a, Dortmund 44227, GermanyTaros Chemicals GmbH & Co KG, Emil-Figge-Str. 76a, Dortmund 44227, GermanyBiochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, Luleå SE-97187, SwedenBiochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, Luleå SE-97187, Sweden; Corresponding author.Three novel feruloyl esterases (Fae125, Fae7262 and Fae68) from Talaromyces wortmannii overexpressed in the C1 platform were evaluated for the transesterification of vinyl ferulate with two acceptors of different size and lipophilicity (prenol and L-arabinose) in detergentless microemulsions. The effect of reaction conditions such as the microemulsion composition, the substrate concentration, the enzyme load, the pH, the temperature and the agitation were investigated. The type A Fae125 belonging to the subfamily 5 (SF5) of phylogenetic classification showed highest yields for the synthesis of both products after optimization of reaction conditions: 81.8% for prenyl ferulate and 33.0% for L-arabinose ferulate. After optimization, an 8-fold increase in the yield and a 12-fold increase in selectivity were achieved for the synthesis of prenyl ferulate.http://www.sciencedirect.com/science/article/pii/S2001037018300941
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Io Antonopoulou Laura Iancu Peter Jütten Alexander Piechot Ulrika Rova Paul Christakopoulos
spellingShingle	Io Antonopoulou Laura Iancu Peter Jütten Alexander Piechot Ulrika Rova Paul Christakopoulos Optimized Enzymatic Synthesis of Feruloyl Derivatives Catalyzed by Three Novel Feruloyl Esterases from Talaromyces wortmannii in Detergentless Microemulsions Computational and Structural Biotechnology Journal
author_facet	Io Antonopoulou Laura Iancu Peter Jütten Alexander Piechot Ulrika Rova Paul Christakopoulos
author_sort	Io Antonopoulou
title	Optimized Enzymatic Synthesis of Feruloyl Derivatives Catalyzed by Three Novel Feruloyl Esterases from Talaromyces wortmannii in Detergentless Microemulsions
title_short	Optimized Enzymatic Synthesis of Feruloyl Derivatives Catalyzed by Three Novel Feruloyl Esterases from Talaromyces wortmannii in Detergentless Microemulsions
title_full	Optimized Enzymatic Synthesis of Feruloyl Derivatives Catalyzed by Three Novel Feruloyl Esterases from Talaromyces wortmannii in Detergentless Microemulsions
title_fullStr	Optimized Enzymatic Synthesis of Feruloyl Derivatives Catalyzed by Three Novel Feruloyl Esterases from Talaromyces wortmannii in Detergentless Microemulsions
title_full_unstemmed	Optimized Enzymatic Synthesis of Feruloyl Derivatives Catalyzed by Three Novel Feruloyl Esterases from Talaromyces wortmannii in Detergentless Microemulsions
title_sort	optimized enzymatic synthesis of feruloyl derivatives catalyzed by three novel feruloyl esterases from talaromyces wortmannii in detergentless microemulsions
publisher	Elsevier
series	Computational and Structural Biotechnology Journal
issn	2001-0370
publishDate	2018-01-01
description	Three novel feruloyl esterases (Fae125, Fae7262 and Fae68) from Talaromyces wortmannii overexpressed in the C1 platform were evaluated for the transesterification of vinyl ferulate with two acceptors of different size and lipophilicity (prenol and L-arabinose) in detergentless microemulsions. The effect of reaction conditions such as the microemulsion composition, the substrate concentration, the enzyme load, the pH, the temperature and the agitation were investigated. The type A Fae125 belonging to the subfamily 5 (SF5) of phylogenetic classification showed highest yields for the synthesis of both products after optimization of reaction conditions: 81.8% for prenyl ferulate and 33.0% for L-arabinose ferulate. After optimization, an 8-fold increase in the yield and a 12-fold increase in selectivity were achieved for the synthesis of prenyl ferulate.
url	http://www.sciencedirect.com/science/article/pii/S2001037018300941
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Optimized Enzymatic Synthesis of Feruloyl Derivatives Catalyzed by Three Novel Feruloyl Esterases from Talaromyces wortmannii in Detergentless Microemulsions

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