Expression optimization, purification, and functional characterization of cholesterol oxidase from Chromobacterium sp. DS1.
Cholesterol oxidase is a bifunctional bacterial flavoenzyme which catalyzes oxidation and isomerization of cholesterol. This valuable enzyme has attracted a great deal of attention because of its wide application in the clinical laboratory, synthesis of steroid derived drugs, food industries, and it...
Main Authors: | Aliakbar Fazaeli, Abolfazl Golestani, Mostafa Lakzaei, Samaneh Sadat Rasi Varaei, Mahdi Aminian |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2019-01-01
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Series: | PLoS ONE |
Online Access: | https://doi.org/10.1371/journal.pone.0212217 |
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