Isolation and Characterization of Bacterial Isolates from Psidium guajava Obtained from Local Markets of Patna and Their Antibiotic Sensitivity Test

• Main Author: Deepak Kumar Jha, Ritu Raj, Pravritti, Samiksha, Aditi, Gulistan Parveen and Niti Yashvardhini
• Format: Article
• Language: English
• Published: Technoscience Publications 2020-12-01
• Series: Nature Environment and Pollution Technology
• Subjects: psidium guajava, antibiotic resistant bacteria, microbiological test, bacterial isolates
• Online Access: http://neptjournal.com/upload-images/(7)B-3691.pdf
• ISSN: 0972-6268; 2395-3454

The study was conducted to investigate the presence of antibiotic resistant bacteria in guava, sold in the local markets of Patna. A total of twenty five fresh samples of guava were collected from five different market places in Patna city. Several microbiological tests were performed to assess the growth and type of bacteria. The colonies were isolated and identified as isolate 1 and 3 (identical to Staphylococcus spp.), isolate 2 (identical to Escherichia spp.) and isolate 4 and 5 (identical to Bacillus spp.) based on their cultural, morphological, Gram’s staining and biochemical characteristics. Antibiotic susceptibility tests were conducted to detect their actual resistance capability. In the present study, S1 and S3 were found resistant to ampicillin, chloramphenicol, ofloxacin and intermediate to gentamycin while S2 was found resistant to ampicillin, chloramphenicol, gentamycin, ciprofloxacin, cephalexin and intermediate to ofloxacin. The isolates S4 and S5 were found sensitive to gentamycin, intermediate to chloramphenicol and resistant to ciprofloxacin. Results of this study showed that the guava samples obtained from different markets of Patna possess multidrug resistant bacteria.