Identification of Major Active Ingredients Responsible for Burn Wound Healing of Centella asiatica Herbs
Centella asiatica herbs have been prescribed as a traditional medicine for wound healing in China and Southeast Asia for a long time. They contain many kinds of triterpenoid compounds, mainly including glycosides (asiaticoside and madecassoside) and corresponding aglycones (asiatic acid and madecass...
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Online Access: | http://dx.doi.org/10.1155/2012/848093 |
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doaj-e95af4dee0224d919d7b841808ed97792020-11-24T22:37:36ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882012-01-01201210.1155/2012/848093848093Identification of Major Active Ingredients Responsible for Burn Wound Healing of Centella asiatica HerbsFang Wu0Difei Bian1Yufeng Xia2Zhunan Gong3Qian Tan4Jiaojiao Chen5Yue Dai6State Key Laboratory of Natural Medicines, Department of Pharmacology Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, ChinaState Key Laboratory of Natural Medicines, Department of Pharmacology Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, ChinaState Key Laboratory of Natural Medicines, Department of Pharmacology Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, ChinaCenter for New Drug Research and Development, College of Life Science, Nanjing Normal University, Nanjing 210024, ChinaDepartment of Burns and Plastic Surgery, Nanjing Drum Tower Hospital, Medical School of Nanjing University, Nanjing 210008, ChinaState Key Laboratory of Natural Medicines, Department of Pharmacology Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, ChinaState Key Laboratory of Natural Medicines, Department of Pharmacology Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, ChinaCentella asiatica herbs have been prescribed as a traditional medicine for wound healing in China and Southeast Asia for a long time. They contain many kinds of triterpenoid compounds, mainly including glycosides (asiaticoside and madecassoside) and corresponding aglycones (asiatic acid and madecassic acid). To identify which is the major active constituent, a comprehensive and comparative study of these compounds was performed. In vitro, primary human skin fibroblasts, originating from healthy human foreskin samples, were treated with various concentrations of asiaticoside, madecassoside, asiatic acid, and madecassic acid, respectively. Cell proliferation, collagen synthesis, MMP-1/TIMP-1 balance, and TGF-β/Smad signaling pathway were investigated. In vivo, mice were orally administered with the four compounds mentioned above for two weeks after burn injury. The speed and quality of wound healing, as well as TGF-β1 levels in skin tissues, were examined. Interestingly, in contrast to prevalent postulations, asiaticoside and madecassoside themselves, rather than their corresponding metabolites asiatic acid and madecassic acid, are recognized as the main active constituents of C. asiatica herbs responsible for burn wound healing. Furthermore, madecassoside is more effective than asiaticoside (P=0.0446 for procollagen type III synthesis in vitro, P=0.0057 for wound healing speed, and P=0.0491 for wound healing pattern in vivo, correspondingly).http://dx.doi.org/10.1155/2012/848093 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fang Wu Difei Bian Yufeng Xia Zhunan Gong Qian Tan Jiaojiao Chen Yue Dai |
spellingShingle |
Fang Wu Difei Bian Yufeng Xia Zhunan Gong Qian Tan Jiaojiao Chen Yue Dai Identification of Major Active Ingredients Responsible for Burn Wound Healing of Centella asiatica Herbs Evidence-Based Complementary and Alternative Medicine |
author_facet |
Fang Wu Difei Bian Yufeng Xia Zhunan Gong Qian Tan Jiaojiao Chen Yue Dai |
author_sort |
Fang Wu |
title |
Identification of Major Active Ingredients Responsible for Burn Wound Healing of Centella asiatica Herbs |
title_short |
Identification of Major Active Ingredients Responsible for Burn Wound Healing of Centella asiatica Herbs |
title_full |
Identification of Major Active Ingredients Responsible for Burn Wound Healing of Centella asiatica Herbs |
title_fullStr |
Identification of Major Active Ingredients Responsible for Burn Wound Healing of Centella asiatica Herbs |
title_full_unstemmed |
Identification of Major Active Ingredients Responsible for Burn Wound Healing of Centella asiatica Herbs |
title_sort |
identification of major active ingredients responsible for burn wound healing of centella asiatica herbs |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2012-01-01 |
description |
Centella asiatica herbs have been prescribed as a traditional medicine for wound healing in China and Southeast Asia for a long time. They contain many kinds of triterpenoid compounds, mainly including glycosides (asiaticoside and madecassoside) and corresponding aglycones (asiatic acid and madecassic acid). To identify which is the major active constituent, a comprehensive and comparative study of these compounds was performed. In vitro, primary human skin fibroblasts, originating from healthy human foreskin samples, were treated with various concentrations of asiaticoside, madecassoside, asiatic acid, and madecassic acid, respectively. Cell proliferation, collagen synthesis, MMP-1/TIMP-1 balance, and TGF-β/Smad signaling pathway were investigated. In vivo, mice were orally administered with the four compounds mentioned above for two weeks after burn injury. The speed and quality of wound healing, as well as TGF-β1 levels in skin tissues, were examined. Interestingly, in contrast to prevalent postulations, asiaticoside and madecassoside themselves, rather than their corresponding metabolites asiatic acid and madecassic acid, are recognized as the main active constituents of C. asiatica herbs responsible for burn wound healing. Furthermore, madecassoside is more effective than asiaticoside (P=0.0446 for procollagen type III synthesis in vitro, P=0.0057 for wound healing speed, and P=0.0491 for wound healing pattern in vivo, correspondingly). |
url |
http://dx.doi.org/10.1155/2012/848093 |
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