Prevalence of bla-CTX-M, bla-SHV, and bla-TEM Genes and Comparison of Antibiotic Resistance Pattern in Extended-spectrum β-lactamase producing and non-producing groups of Klebsiella pneumoniae Isolated from Clinical Samples in Kerman Hospitals

• Main Authors: Mohammad Moradi, Amin Norouzi, Majid Taati moghadam
• Format: Article
• Language: fas
• Published: Fasa University of Medical Sciences 2016-04-01
• Series: Journal of Fasa University of Medical Sciences
• Subjects: Klebsiella pneumonia; Antibiotic resistance; β- lactamase genes; PCR
• Online Access: http://journal.fums.ac.ir/browse.php?a_code=A-10-1010-1&slc_lang=en&sid=1
• ISSN: 2228-5105; 2228-7329

Background & Objectives: Antibiotic resistance among pathogens bacteria are an important problem noted worldwide. Beta-lactamases that are produced by Enterobacteriaceae have been located mainly on plasmid. Treatment of these bacterial infections which produced β-lactamase are a major problem. Materials & Methods: 111 Klebsiella pneumoniae isolates were collected from hospitals in Kerman; therefore, antibiotic susceptibility test was performed by disk diffusion method. At first, detection of ESBLs were performed by phenotypic confirmatory test, and presence of bla-SHV, bla-TEM, and bla-CTX-M were detected by PCR. Results: Resistant to ampicillin (92.5%) was more than others antibiotics, and the imipenem (89%) was the most effective antibiotic against Klebsiella pneumoniae isolates. Additionally, the resistance to all antibiotics in ESBLs-producing Klebsiella pneumoniae was more than that of Non-ESBL Klebsiella pneumoniae. After the detection of bla-SHV, bla-TEM, and bla-CTX-M genes in Klebsiella pneumoniae by PCR, 56 (50.4%) isolates presented these genes. Conclusion: With regard to high prevalence of ESBLs genes and high level of antibiotic resistance in bacteria, detecting these genes can prevent the extension of antibiotic resistance through these bacteria.