Transcripts of antibacterial peptides in chicken erythrocytes infected with Marek’s disease virus

• Main Authors: Sheng Niu, Ali Raza Jahejo, Fa-jie Jia, Xin Li, Guan-bao Ning, Ding Zhang, Hai-li Ma, Wei-fang Hao, Wen-wei Gao, Yu-jun Zhao, Shi-min Gao, Gui-lan Li, Jian-hui Li, Fang Yan, Rong-kun Gao, Yu-hai Bi, Ling-xia Han, George F. Gao, Wen-xia Tian
• Format: Article
• Language: English
• Published: BMC 2018-11-01
• Series: BMC Veterinary Research
• Subjects: Chicken; Erythrocytes; AvBDs; TLRs; MDV
• Online Access: http://link.springer.com/article/10.1186/s12917-018-1678-7

Abstract Background Chicken erythrocytes are involved in immunity through binding of toll-like receptors (TLRs) with their ligands to activate downstream signaling and lead to cytokine production in erythrocytes. Some avian β-defensins (AvBDs) are constitutively expressed in tissues and some others can be induced by various bacteria and viruses. However, the expression of AvBDs in erythrocytes has not yet been studied extensively. Results The transcripts of eight AvBDs (AvBD1 to AvBD7, and AvBD9) and liver-expressed antimicrobial peptide-2 (LEAP-2) were found in normal chicken erythrocytes. The expression levels of AvBD2, 4 and 7 were significantly increased (P < 0.01), whereas the levels of AvBD1, 6 and 9 were significantly decreased (P < 0.01) after Marek’s disease virus (MDV) infection. The mRNA expression level of LEAP-2 was not significantly changed after MDV infection. Highest viral nucleic acid (VNA) of MDV in the feather tips among the tested time points was found at 14 days post-infection (d.p.i.). In addition, 35 MD5-related gene segments were detected in the erythrocytes at 14 d.p.i. by transcriptome sequencing. Conclusions These results suggest that the AvBDs in chicken erythrocytes may participate in MDV-induced host immune responses.