Genotoxic effect of ethephon on the root meristems of Allium cepa L.
Genotoxic effect of ethephon [(2-chloroethyl) phosphonic acid], a PGR that has replaced calcium carbide as an agent for fruit ripening, was studied by using a root chromosome assay. Onion root meristems were cultured and analyzed after exposure with ethephon at concentrations of 200 ppm and 500 ppm...
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Communications in Plant Sciences
2014-04-01
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doaj-e9047e443ab84a37806ce35e1a40deb82020-11-25T00:19:20ZengCommunications in Plant SciencesCommunications in Plant Sciences2237-40272237-40272014-04-0141-21922Genotoxic effect of ethephon on the root meristems of Allium cepa L.Joyce Sudandara Priya0Preethi Purushothaman1Cordilea Hannah2Sarah Matthew 3Sowmya Thomas4Madras Christian College, IndiaMadras Christian College, IndiaWomen’s Christian College, IndiaMadras Christian College, IndiaMadras Christian College, IndiaGenotoxic effect of ethephon [(2-chloroethyl) phosphonic acid], a PGR that has replaced calcium carbide as an agent for fruit ripening, was studied by using a root chromosome assay. Onion root meristems were cultured and analyzed after exposure with ethephon at concentrations of 200 ppm and 500 ppm for 2, 24, and 48 h. There was a time dependent decrease and an immediate toxic effect on dividing cells in mitotic index when compared to control (the mitotic indices of root meristems treated with 200 ppm ethephon for 2, 24, and 48 h were 11.1%, 10.7%, and 8.9%, respectively; and with 500 ppm ethephon for 2, 24, and 48 h the mitotic indices were 10.8%, 10.2%, and 9.0% respectively). The total chromosomal aberrations increased with prolonged exposures indicating duration dependent effect compared to control (the chromosomal aberrations of root meristems treated with 200 ppm ethephon for 2, 24, and 48 h were 14.5%, 15.6%, and 34.0% respectively; and with 500 ppm ethephon for 2, 24, and 48 h the chromosomal aberrations were 20.6%, 26.1%, and 39.8%, respectively). Chromosomal anomalies such as clumps and breaks were of frequent occurrence. This study proves the genotoxic effect of ethephon and emphasizes the judicial use of this compound as a PGR on edible plants and plant parts.http://complantsci.files.wordpress.com/2014/04/complantsci_4_1_3.pdfCytotoxicityChromosomal anomaliesOnion root meristems |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Joyce Sudandara Priya Preethi Purushothaman Cordilea Hannah Sarah Matthew Sowmya Thomas |
spellingShingle |
Joyce Sudandara Priya Preethi Purushothaman Cordilea Hannah Sarah Matthew Sowmya Thomas Genotoxic effect of ethephon on the root meristems of Allium cepa L. Communications in Plant Sciences Cytotoxicity Chromosomal anomalies Onion root meristems |
author_facet |
Joyce Sudandara Priya Preethi Purushothaman Cordilea Hannah Sarah Matthew Sowmya Thomas |
author_sort |
Joyce Sudandara Priya |
title |
Genotoxic effect of ethephon on the root meristems of Allium cepa L. |
title_short |
Genotoxic effect of ethephon on the root meristems of Allium cepa L. |
title_full |
Genotoxic effect of ethephon on the root meristems of Allium cepa L. |
title_fullStr |
Genotoxic effect of ethephon on the root meristems of Allium cepa L. |
title_full_unstemmed |
Genotoxic effect of ethephon on the root meristems of Allium cepa L. |
title_sort |
genotoxic effect of ethephon on the root meristems of allium cepa l. |
publisher |
Communications in Plant Sciences |
series |
Communications in Plant Sciences |
issn |
2237-4027 2237-4027 |
publishDate |
2014-04-01 |
description |
Genotoxic effect of ethephon [(2-chloroethyl) phosphonic acid], a PGR that has replaced calcium carbide as an agent for fruit ripening, was studied by using a root chromosome assay. Onion root meristems were cultured and analyzed after exposure with ethephon at concentrations of 200 ppm and 500 ppm for 2, 24, and 48 h. There was a time dependent decrease and an immediate toxic effect on dividing cells in mitotic index when compared to control (the mitotic indices of root meristems treated with 200 ppm ethephon for 2, 24, and 48 h were 11.1%, 10.7%, and 8.9%, respectively; and with 500 ppm ethephon for 2, 24, and 48 h the mitotic indices were 10.8%, 10.2%, and 9.0% respectively). The total chromosomal aberrations increased with prolonged exposures indicating duration dependent effect compared to control (the chromosomal aberrations of root meristems treated with 200 ppm ethephon for 2, 24, and 48 h were 14.5%, 15.6%, and 34.0% respectively; and with 500 ppm ethephon for 2, 24, and 48 h the chromosomal aberrations were 20.6%, 26.1%, and 39.8%, respectively). Chromosomal anomalies such as clumps and breaks were of frequent occurrence. This study proves the genotoxic effect of ethephon and emphasizes the judicial use of this compound as a PGR on edible plants and plant parts. |
topic |
Cytotoxicity Chromosomal anomalies Onion root meristems |
url |
http://complantsci.files.wordpress.com/2014/04/complantsci_4_1_3.pdf |
work_keys_str_mv |
AT joycesudandarapriya genotoxiceffectofethephonontherootmeristemsofalliumcepal AT preethipurushothaman genotoxiceffectofethephonontherootmeristemsofalliumcepal AT cordileahannah genotoxiceffectofethephonontherootmeristemsofalliumcepal AT sarahmatthew genotoxiceffectofethephonontherootmeristemsofalliumcepal AT sowmyathomas genotoxiceffectofethephonontherootmeristemsofalliumcepal |
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