The Photodynamic Properties and the Genotoxicity of Heat-Treated Silicalite-1 Films

• Main Authors: Ivan Jirka, Ivana Kopová, Pavel Kubát, Edyta Tabor, Lucie Bačáková, Milan Bouša, Petr Sajdl
• Format: Article
• Language: English
• Published: MDPI AG 2019-02-01
• Series: Materials
• Subjects: genotoxicity; surface coating; implant material; singlet oxygen
• Online Access: https://www.mdpi.com/1996-1944/12/4/567

We investigated the use of a supported silicalite-1 film (<b><i>SF</i></b>) as a promising coating for metallic materials used in the fabrication of prostheses. The role of carbonaceous residua present on high-temperature calcined-<b><i>SF</i></b> in generating singlet oxygen for future use as a sterilization method has also been addressed, and the potential genotoxicity of these residua in osteoblast-like cells has been investigated. Calcination of as-synthesized <b><i>SF</i></b> induced the appearance of a rather complicated mixture of aliphatic and aromatic species on its outer surface. A series of variously volatile polycyclic aromatic hydrocarbons (PAH), including naphthalene, fluorene, phenanthrene, anthracene, fluoranthene, and pyrene, were identified in micromole concentrations. Irradiation of these PAHs on calcined-<b><i>SF</i></b> immersed in air-saturated chloroform led to the formation of very low concentrations of singlet oxygen. However, an increased level of DNA damage was observed on calcined-SF by immunofluorescence staining of phosphorylated histone H2AX analyzed by flow cytometry.