Identification of milk protein percentage QTLs in Italian Friesian cattle by selective genotyping two GDD families with AFLP and SSR markers

• Main Authors: R. Negrini, R. Mazza, F. Schiavini, E. Milanesi, P. Ajmone-Marsan
• Format: Article
• Language: English
• Published: Taylor & Francis Group 2010-04-01
• Series: Italian Journal of Animal Science
• Subjects: Quantitative trait loci, Molecular markers, Cattle, Milk quality.
• Online Access: http://www.aspajournal.it/index.php/ijas/article/view/1350

In this work we evaluated the combined use of AFLP markers and selective genotyping in a Grand Daughter experimental design (GDD), to identify chromosomal regions candidate to contain QTL for milk protein percentage (PP) in Italian Friesian cattle. We verified the approach by microsatellite analysis of 4 candidate chromosomal regions. Twenty-five AFLP primer combinations, assayed on the extreme minus and plus-variant half-sibs and on the grandsires of 2 GDD families, produced 180 polymorphic bands of paternal origin. Association analysis resulted in 16 AFLPs significantly associated to PP-EBVs (P£0.05), that mapped on 8 chromosomes, in regions where PP-QTLs were identified in previous studies. Microsatellite analysis confirmed association for 2 regions of BTA10 and BTA28.