A criterion of the vertical spacing between fluorescent molecules in a waveguide structure

We demonstrate a method to discriminate the vertical spacing of fluorescent molecules in a guided mode structure. A function that depends only on depth and wavelength is constructed to detect the axial position of a pair of fluorescent molecules, which is especially suitable for the measurement of m...

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Main Authors:	Yikai Chen, Lu Zhang, Binxuan Ding, Fulian Chen
Format:	Article
Language:	English
Published:	Elsevier 2020-06-01
Series:	Results in Physics
Subjects:	Leakage radiation microscopy Molecular spacing Surface plasmons Guided wave coupling emission
Online Access:	http://www.sciencedirect.com/science/article/pii/S2211379720303880

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spelling	doaj-e85a72fa5c5e48a08358b7e8643495842020-11-25T03:52:12ZengElsevierResults in Physics2211-37972020-06-0117103030A criterion of the vertical spacing between fluorescent molecules in a waveguide structureYikai Chen0Lu Zhang1Binxuan Ding2Fulian Chen3Corresponding author.; School of Sciences, Nanjing University of Science and Technology, Nanjing 210094, ChinaSchool of Sciences, Nanjing University of Science and Technology, Nanjing 210094, ChinaSchool of Sciences, Nanjing University of Science and Technology, Nanjing 210094, ChinaSchool of Sciences, Nanjing University of Science and Technology, Nanjing 210094, ChinaWe demonstrate a method to discriminate the vertical spacing of fluorescent molecules in a guided mode structure. A function that depends only on depth and wavelength is constructed to detect the axial position of a pair of fluorescent molecules, which is especially suitable for the measurement of microstructure gaps and is not affected by the diffraction limit. Since directional fluorescence radiation information can be collected from the back focal plane in a leakage radiation microscope, we can utilize the appropriate emission angles and corresponding waveguide modes. The relative position of the fluorescent molecules is calculated by analyzing the intensity ratio between modes emission, so as to determine the vertical distance between the molecules.http://www.sciencedirect.com/science/article/pii/S2211379720303880Leakage radiation microscopyMolecular spacingSurface plasmonsGuided wave coupling emission
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Yikai Chen Lu Zhang Binxuan Ding Fulian Chen
spellingShingle	Yikai Chen Lu Zhang Binxuan Ding Fulian Chen A criterion of the vertical spacing between fluorescent molecules in a waveguide structure Results in Physics Leakage radiation microscopy Molecular spacing Surface plasmons Guided wave coupling emission
author_facet	Yikai Chen Lu Zhang Binxuan Ding Fulian Chen
author_sort	Yikai Chen
title	A criterion of the vertical spacing between fluorescent molecules in a waveguide structure
title_short	A criterion of the vertical spacing between fluorescent molecules in a waveguide structure
title_full	A criterion of the vertical spacing between fluorescent molecules in a waveguide structure
title_fullStr	A criterion of the vertical spacing between fluorescent molecules in a waveguide structure
title_full_unstemmed	A criterion of the vertical spacing between fluorescent molecules in a waveguide structure
title_sort	criterion of the vertical spacing between fluorescent molecules in a waveguide structure
publisher	Elsevier
series	Results in Physics
issn	2211-3797
publishDate	2020-06-01
description	We demonstrate a method to discriminate the vertical spacing of fluorescent molecules in a guided mode structure. A function that depends only on depth and wavelength is constructed to detect the axial position of a pair of fluorescent molecules, which is especially suitable for the measurement of microstructure gaps and is not affected by the diffraction limit. Since directional fluorescence radiation information can be collected from the back focal plane in a leakage radiation microscope, we can utilize the appropriate emission angles and corresponding waveguide modes. The relative position of the fluorescent molecules is calculated by analyzing the intensity ratio between modes emission, so as to determine the vertical distance between the molecules.
topic	Leakage radiation microscopy Molecular spacing Surface plasmons Guided wave coupling emission
url	http://www.sciencedirect.com/science/article/pii/S2211379720303880
work_keys_str_mv	AT yikaichen acriterionoftheverticalspacingbetweenfluorescentmoleculesinawaveguidestructure AT luzhang acriterionoftheverticalspacingbetweenfluorescentmoleculesinawaveguidestructure AT binxuanding acriterionoftheverticalspacingbetweenfluorescentmoleculesinawaveguidestructure AT fulianchen acriterionoftheverticalspacingbetweenfluorescentmoleculesinawaveguidestructure AT yikaichen criterionoftheverticalspacingbetweenfluorescentmoleculesinawaveguidestructure AT luzhang criterionoftheverticalspacingbetweenfluorescentmoleculesinawaveguidestructure AT binxuanding criterionoftheverticalspacingbetweenfluorescentmoleculesinawaveguidestructure AT fulianchen criterionoftheverticalspacingbetweenfluorescentmoleculesinawaveguidestructure
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A criterion of the vertical spacing between fluorescent molecules in a waveguide structure

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