Detection of Tumorigenic Rhizobia in Asymptomatic Peach Plants by PCR

Detection of Tumorigenic Rhizobia in Asymptomatic Peach Plants by PCR

At present the only method for the detection of Rhizobium radiobacter and R. rhizogenes (ex Agrobacterium tumefaciens) in plants is by isolating the bacteria on selective medium and testing them in vivo for pathogenicity. This procedure is time-consuming and not appropriate for detecting low concent...

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Main Authors: A. Raio, G. Puopolo, R. Peluso, L. Cozzolino, A. Zoina
Format: Article
Language:English
Published: Firenze University Press 2004-08-01
Series:Phytopathologia Mediterranea
Online Access:https://oajournals.fupress.net/index.php/pm/article/view/5058
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spelling doaj-e835b4785548442cac59476e1e29e9902020-11-25T03:43:49ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952004-08-0143210.14601/Phytopathol_Mediterr-17441740Detection of Tumorigenic Rhizobia in Asymptomatic Peach Plants by PCRA. RaioG. PuopoloR. PelusoL. CozzolinoA. ZoinaAt present the only method for the detection of Rhizobium radiobacter and R. rhizogenes (ex Agrobacterium tumefaciens) in plants is by isolating the bacteria on selective medium and testing them in vivo for pathogenicity. This procedure is time-consuming and not appropriate for detecting low concentrations of these agrobacteria. In this study a protocol was developed for detecting tumorigenic agrobacteria in the stem and root tissues of artificially inoculated peach plants. DNA was extracted from the plant tissues by a rapid procedure and then a 246 bp sequence of the vir region of the pTi was amplified by PCR. The target sequence was found in all stem and root samples of asymptomatic peach plants, and was evidenced in all the samples analyzed showing the effectiveness and reliability of the method.https://oajournals.fupress.net/index.php/pm/article/view/5058
collection DOAJ
language English
format Article
sources DOAJ
author A. Raio
G. Puopolo
R. Peluso
L. Cozzolino
A. Zoina
spellingShingle A. Raio
G. Puopolo
R. Peluso
L. Cozzolino
A. Zoina
Detection of Tumorigenic Rhizobia in Asymptomatic Peach Plants by PCR
Phytopathologia Mediterranea
author_facet A. Raio
G. Puopolo
R. Peluso
L. Cozzolino
A. Zoina
author_sort A. Raio
title Detection of Tumorigenic Rhizobia in Asymptomatic Peach Plants by PCR
title_short Detection of Tumorigenic Rhizobia in Asymptomatic Peach Plants by PCR
title_full Detection of Tumorigenic Rhizobia in Asymptomatic Peach Plants by PCR
title_fullStr Detection of Tumorigenic Rhizobia in Asymptomatic Peach Plants by PCR
title_full_unstemmed Detection of Tumorigenic Rhizobia in Asymptomatic Peach Plants by PCR
title_sort detection of tumorigenic rhizobia in asymptomatic peach plants by pcr
publisher Firenze University Press
series Phytopathologia Mediterranea
issn 0031-9465
1593-2095
publishDate 2004-08-01
description At present the only method for the detection of Rhizobium radiobacter and R. rhizogenes (ex Agrobacterium tumefaciens) in plants is by isolating the bacteria on selective medium and testing them in vivo for pathogenicity. This procedure is time-consuming and not appropriate for detecting low concentrations of these agrobacteria. In this study a protocol was developed for detecting tumorigenic agrobacteria in the stem and root tissues of artificially inoculated peach plants. DNA was extracted from the plant tissues by a rapid procedure and then a 246 bp sequence of the vir region of the pTi was amplified by PCR. The target sequence was found in all stem and root samples of asymptomatic peach plants, and was evidenced in all the samples analyzed showing the effectiveness and reliability of the method.
url https://oajournals.fupress.net/index.php/pm/article/view/5058
work_keys_str_mv AT araio detectionoftumorigenicrhizobiainasymptomaticpeachplantsbypcr
AT gpuopolo detectionoftumorigenicrhizobiainasymptomaticpeachplantsbypcr
AT rpeluso detectionoftumorigenicrhizobiainasymptomaticpeachplantsbypcr
AT lcozzolino detectionoftumorigenicrhizobiainasymptomaticpeachplantsbypcr
AT azoina detectionoftumorigenicrhizobiainasymptomaticpeachplantsbypcr
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