A Comprehensive Study of Cutaneous Fibropapillomatosis in Free-Ranging Roe Deer (<i>Capreolus capreolus</i>) and Red Deer (<i>Cervus elaphus</i>): from Clinical Manifestations to Whole-Genome Sequencing of Papillomaviruses

A Comprehensive Study of Cutaneous Fibropapillomatosis in Free-Ranging Roe Deer (<i>Capreolus capreolus</i>) and Red Deer (<i>Cervus elaphus</i>): from Clinical Manifestations to Whole-Genome Sequencing of Papillomaviruses

Papillomaviruses (PVs) are an extremely large group of viruses that cause skin and mucosa infections in humans and various animals. In roe deer and red deer, most PVs belong to the <i>Deltapapillomavirus</i> genus and cause neoplastic changes that are generally described as fibropapillom...

Full description

Bibliographic Details
Main Authors: Jernej Kmetec, Urška Kuhar, Aleksandra Grilc Fajfar, Diana Žele Vengušt, Gorazd Vengušt
Format: Article
Language:English
Published: MDPI AG 2020-09-01
Series:Viruses
Subjects:
papillomavirus
fibropapillomatosis
red deer
roe deer
genome
NGS
Online Access:https://www.mdpi.com/1999-4915/12/9/1001
id doaj-e8000a67527749878aff5f373d16359b
record_format Article
spelling doaj-e8000a67527749878aff5f373d16359b2020-11-25T03:33:33ZengMDPI AGViruses1999-49152020-09-01121001100110.3390/v12091001A Comprehensive Study of Cutaneous Fibropapillomatosis in Free-Ranging Roe Deer (<i>Capreolus capreolus</i>) and Red Deer (<i>Cervus elaphus</i>): from Clinical Manifestations to Whole-Genome Sequencing of PapillomavirusesJernej Kmetec0Urška Kuhar1Aleksandra Grilc Fajfar2Diana Žele Vengušt3Gorazd Vengušt4Institute of Pathology, Wild Animals, Fish and Bees, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, SloveniaInstitute of Microbiology and Parasitology, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, SloveniaInstitute of Microbiology and Parasitology, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, SloveniaInstitute of Pathology, Wild Animals, Fish and Bees, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, SloveniaInstitute of Pathology, Wild Animals, Fish and Bees, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, SloveniaPapillomaviruses (PVs) are an extremely large group of viruses that cause skin and mucosa infections in humans and various animals. In roe deer and red deer, most PVs belong to the <i>Deltapapillomavirus</i> genus and cause neoplastic changes that are generally described as fibropapillomas. Despite the wide distribution of roe and red deer throughout Europe and beyond, the data in the scientific literature regarding the widespread distribution of PVs and the genetic variability of PV genomes in these species are rather scarce. This study describes cutaneous fibropapillomatosis cases in roe and red deer with clinical manifestations that are typical of infections with PVs. In all cases, the presence of PV DNA was confirmed using PCR, followed by Sanger sequencing of the partial <i>L1</i> gene. The complete PV genomes were determined in all the investigated samples using next-generation sequencing technology, revealing infections of roe deer with the CcaPV1-type and red deer with the CePV1v-type variant. A comparison of the complete CcaPV1-type and CePV1v-type variant genome sequences reported here with already available complete genome sequences in GenBank revealed their great genetic stability across time and space.https://www.mdpi.com/1999-4915/12/9/1001papillomavirusfibropapillomatosisred deerroe deergenomeNGS
collection DOAJ
language English
format Article
sources DOAJ
author Jernej Kmetec
Urška Kuhar
Aleksandra Grilc Fajfar
Diana Žele Vengušt
Gorazd Vengušt
spellingShingle Jernej Kmetec
Urška Kuhar
Aleksandra Grilc Fajfar
Diana Žele Vengušt
Gorazd Vengušt
A Comprehensive Study of Cutaneous Fibropapillomatosis in Free-Ranging Roe Deer (<i>Capreolus capreolus</i>) and Red Deer (<i>Cervus elaphus</i>): from Clinical Manifestations to Whole-Genome Sequencing of Papillomaviruses
Viruses
papillomavirus
fibropapillomatosis
red deer
roe deer
genome
NGS
author_facet Jernej Kmetec
Urška Kuhar
Aleksandra Grilc Fajfar
Diana Žele Vengušt
Gorazd Vengušt
author_sort Jernej Kmetec
title A Comprehensive Study of Cutaneous Fibropapillomatosis in Free-Ranging Roe Deer (<i>Capreolus capreolus</i>) and Red Deer (<i>Cervus elaphus</i>): from Clinical Manifestations to Whole-Genome Sequencing of Papillomaviruses
title_short A Comprehensive Study of Cutaneous Fibropapillomatosis in Free-Ranging Roe Deer (<i>Capreolus capreolus</i>) and Red Deer (<i>Cervus elaphus</i>): from Clinical Manifestations to Whole-Genome Sequencing of Papillomaviruses
title_full A Comprehensive Study of Cutaneous Fibropapillomatosis in Free-Ranging Roe Deer (<i>Capreolus capreolus</i>) and Red Deer (<i>Cervus elaphus</i>): from Clinical Manifestations to Whole-Genome Sequencing of Papillomaviruses
title_fullStr A Comprehensive Study of Cutaneous Fibropapillomatosis in Free-Ranging Roe Deer (<i>Capreolus capreolus</i>) and Red Deer (<i>Cervus elaphus</i>): from Clinical Manifestations to Whole-Genome Sequencing of Papillomaviruses
title_full_unstemmed A Comprehensive Study of Cutaneous Fibropapillomatosis in Free-Ranging Roe Deer (<i>Capreolus capreolus</i>) and Red Deer (<i>Cervus elaphus</i>): from Clinical Manifestations to Whole-Genome Sequencing of Papillomaviruses
title_sort comprehensive study of cutaneous fibropapillomatosis in free-ranging roe deer (<i>capreolus capreolus</i>) and red deer (<i>cervus elaphus</i>): from clinical manifestations to whole-genome sequencing of papillomaviruses
publisher MDPI AG
series Viruses
issn 1999-4915
publishDate 2020-09-01
description Papillomaviruses (PVs) are an extremely large group of viruses that cause skin and mucosa infections in humans and various animals. In roe deer and red deer, most PVs belong to the <i>Deltapapillomavirus</i> genus and cause neoplastic changes that are generally described as fibropapillomas. Despite the wide distribution of roe and red deer throughout Europe and beyond, the data in the scientific literature regarding the widespread distribution of PVs and the genetic variability of PV genomes in these species are rather scarce. This study describes cutaneous fibropapillomatosis cases in roe and red deer with clinical manifestations that are typical of infections with PVs. In all cases, the presence of PV DNA was confirmed using PCR, followed by Sanger sequencing of the partial <i>L1</i> gene. The complete PV genomes were determined in all the investigated samples using next-generation sequencing technology, revealing infections of roe deer with the CcaPV1-type and red deer with the CePV1v-type variant. A comparison of the complete CcaPV1-type and CePV1v-type variant genome sequences reported here with already available complete genome sequences in GenBank revealed their great genetic stability across time and space.
topic papillomavirus
fibropapillomatosis
red deer
roe deer
genome
NGS
url https://www.mdpi.com/1999-4915/12/9/1001
work_keys_str_mv AT jernejkmetec acomprehensivestudyofcutaneousfibropapillomatosisinfreerangingroedeericapreoluscapreolusiandreddeericervuselaphusifromclinicalmanifestationstowholegenomesequencingofpapillomaviruses
AT urskakuhar acomprehensivestudyofcutaneousfibropapillomatosisinfreerangingroedeericapreoluscapreolusiandreddeericervuselaphusifromclinicalmanifestationstowholegenomesequencingofpapillomaviruses
AT aleksandragrilcfajfar acomprehensivestudyofcutaneousfibropapillomatosisinfreerangingroedeericapreoluscapreolusiandreddeericervuselaphusifromclinicalmanifestationstowholegenomesequencingofpapillomaviruses
AT dianazelevengust acomprehensivestudyofcutaneousfibropapillomatosisinfreerangingroedeericapreoluscapreolusiandreddeericervuselaphusifromclinicalmanifestationstowholegenomesequencingofpapillomaviruses
AT gorazdvengust acomprehensivestudyofcutaneousfibropapillomatosisinfreerangingroedeericapreoluscapreolusiandreddeericervuselaphusifromclinicalmanifestationstowholegenomesequencingofpapillomaviruses
AT jernejkmetec comprehensivestudyofcutaneousfibropapillomatosisinfreerangingroedeericapreoluscapreolusiandreddeericervuselaphusifromclinicalmanifestationstowholegenomesequencingofpapillomaviruses
AT urskakuhar comprehensivestudyofcutaneousfibropapillomatosisinfreerangingroedeericapreoluscapreolusiandreddeericervuselaphusifromclinicalmanifestationstowholegenomesequencingofpapillomaviruses
AT aleksandragrilcfajfar comprehensivestudyofcutaneousfibropapillomatosisinfreerangingroedeericapreoluscapreolusiandreddeericervuselaphusifromclinicalmanifestationstowholegenomesequencingofpapillomaviruses
AT dianazelevengust comprehensivestudyofcutaneousfibropapillomatosisinfreerangingroedeericapreoluscapreolusiandreddeericervuselaphusifromclinicalmanifestationstowholegenomesequencingofpapillomaviruses
AT gorazdvengust comprehensivestudyofcutaneousfibropapillomatosisinfreerangingroedeericapreoluscapreolusiandreddeericervuselaphusifromclinicalmanifestationstowholegenomesequencingofpapillomaviruses
_version_ 1724563004676112384

Similar Items