A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity
The assembly of the splicesome involves several distinct stages that require the sequential action of DExD/H-box RNA helicases. Here, the authors uncover a new intermediate, the pre-B complex, that accumulates in the presence of an inactive form of the DEAD-box protein Prp28.
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2016-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms11997 |
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doaj-e7d1d18e40824533a67b9650add6c83c2021-05-11T11:06:30ZengNature Publishing GroupNature Communications2041-17232016-07-017111210.1038/ncomms11997A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activityCarsten Boesler0Norbert Rigo1Maria M. Anokhina2Marcel J. Tauchert3Dmitry E. Agafonov4Berthold Kastner5Henning Urlaub6Ralf Ficner7Cindy L. Will8Reinhard Lührmann9Department of Cellular Biochemistry, MPI for Biophysical ChemistryDepartment of Cellular Biochemistry, MPI for Biophysical ChemistryDepartment of Cellular Biochemistry, MPI for Biophysical ChemistryDepartment of Molecular Structural Biology, Institute for Microbiology and Genetics, GZMB, Georg-August-Universität GöttingenDepartment of Cellular Biochemistry, MPI for Biophysical ChemistryDepartment of Cellular Biochemistry, MPI for Biophysical ChemistryBioanalytical Mass Spectrometry Group, MPI for Biophysical ChemistryDepartment of Molecular Structural Biology, Institute for Microbiology and Genetics, GZMB, Georg-August-Universität GöttingenDepartment of Cellular Biochemistry, MPI for Biophysical ChemistryDepartment of Cellular Biochemistry, MPI for Biophysical ChemistryThe assembly of the splicesome involves several distinct stages that require the sequential action of DExD/H-box RNA helicases. Here, the authors uncover a new intermediate, the pre-B complex, that accumulates in the presence of an inactive form of the DEAD-box protein Prp28.https://doi.org/10.1038/ncomms11997 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Carsten Boesler Norbert Rigo Maria M. Anokhina Marcel J. Tauchert Dmitry E. Agafonov Berthold Kastner Henning Urlaub Ralf Ficner Cindy L. Will Reinhard Lührmann |
| spellingShingle |
Carsten Boesler Norbert Rigo Maria M. Anokhina Marcel J. Tauchert Dmitry E. Agafonov Berthold Kastner Henning Urlaub Ralf Ficner Cindy L. Will Reinhard Lührmann A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity Nature Communications |
| author_facet |
Carsten Boesler Norbert Rigo Maria M. Anokhina Marcel J. Tauchert Dmitry E. Agafonov Berthold Kastner Henning Urlaub Ralf Ficner Cindy L. Will Reinhard Lührmann |
| author_sort |
Carsten Boesler |
| title |
A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity |
| title_short |
A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity |
| title_full |
A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity |
| title_fullStr |
A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity |
| title_full_unstemmed |
A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity |
| title_sort |
spliceosome intermediate with loosely associated tri-snrnp accumulates in the absence of prp28 atpase activity |
| publisher |
Nature Publishing Group |
| series |
Nature Communications |
| issn |
2041-1723 |
| publishDate |
2016-07-01 |
| description |
The assembly of the splicesome involves several distinct stages that require the sequential action of DExD/H-box RNA helicases. Here, the authors uncover a new intermediate, the pre-B complex, that accumulates in the presence of an inactive form of the DEAD-box protein Prp28. |
| url |
https://doi.org/10.1038/ncomms11997 |
| work_keys_str_mv |
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